A human histone (H2B.1 variant gene, located on chromosome 1, utilizes alternative 3' end processing

D. Collart, P. L. Romain, K. Huebner, S. Pockwinse, S. Pilapil, L. A. Cannizzaro, J. B. Lian, C. M. Croce, J. L. Stein, G. S. Stein

Research output: Contribution to journalArticle

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Abstract

A variant human H2B histone gene (GL105), previously shown to encode a 2300 nt replication independent mRNA, has been cloned. We demonstrate this gene expresses alternative mRNAs regulated differentially during the HeLa S3 cell cycle. The H2B-GL105 gene encodes both a 500 nt cell cycle dependent mRNA and a 2300 nt constitutively expressed mRNA. The 3' end of the cell cycle regulated mRNA terminates immediately following the region of hyphenated dyad symmetry typical of most histone mRNAs, whereas the constitutively expressed mRNA has a 1798 nt non-translated trailer that contains the same region of hyphenated dyad symmetry but is polyadenylated. The cap site for the H2B-GL105 mRNAs is located 42 nt upstream of the protein coding region. The H2B-GL105 histone gene was localized to chromosome region 1q21-1q23 by chromosomal in situ hybridization and by analysis of rodent-human somatic cell hybrids using an H2B-GL105 specific probe. The H2B-GL105 gene is paired with a functional H2A histone gene and this H2A/H2B gene pair is separated by a bidirectionally transcribed intergenic promoter region containing consensus TATA and CCAAT boxes and an OTF-1 element. These results demonstrate that cell cycle regulated and constitutively expressed histone mRNAs can be encoded by the same gene, and indicate that alternative 3' end processing may be an important mechanism for regulation of histone mRNA. Such control further increases the versatility by which cells can modulate the synthesis of replication-dependent as well as variant histone proteins during the cell cycle and at the onset of differentiation.

Original languageEnglish (US)
Pages (from-to)374-385
Number of pages12
JournalJournal of Cellular Biochemistry
Volume50
Issue number4
DOIs
StatePublished - 1992
Externally publishedYes

Fingerprint

Chromosomes, Human, Pair 1
Chromosomes
Histones
Genes
Messenger RNA
Processing
Cells
Cell Cycle
Cell Cycle Proteins
TATA Box
Intergenic DNA
Light trailers
Hybrid Cells
HeLa Cells
Genetic Promoter Regions
Open Reading Frames
In Situ Hybridization
Rodentia
Proteins

Keywords

  • alternative 3' processing
  • growth regulation
  • histone variant

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology

Cite this

Collart, D., Romain, P. L., Huebner, K., Pockwinse, S., Pilapil, S., Cannizzaro, L. A., ... Stein, G. S. (1992). A human histone (H2B.1 variant gene, located on chromosome 1, utilizes alternative 3' end processing. Journal of Cellular Biochemistry, 50(4), 374-385. https://doi.org/10.1002/jcb.240500406

A human histone (H2B.1 variant gene, located on chromosome 1, utilizes alternative 3' end processing. / Collart, D.; Romain, P. L.; Huebner, K.; Pockwinse, S.; Pilapil, S.; Cannizzaro, L. A.; Lian, J. B.; Croce, C. M.; Stein, J. L.; Stein, G. S.

In: Journal of Cellular Biochemistry, Vol. 50, No. 4, 1992, p. 374-385.

Research output: Contribution to journalArticle

Collart, D, Romain, PL, Huebner, K, Pockwinse, S, Pilapil, S, Cannizzaro, LA, Lian, JB, Croce, CM, Stein, JL & Stein, GS 1992, 'A human histone (H2B.1 variant gene, located on chromosome 1, utilizes alternative 3' end processing', Journal of Cellular Biochemistry, vol. 50, no. 4, pp. 374-385. https://doi.org/10.1002/jcb.240500406
Collart, D. ; Romain, P. L. ; Huebner, K. ; Pockwinse, S. ; Pilapil, S. ; Cannizzaro, L. A. ; Lian, J. B. ; Croce, C. M. ; Stein, J. L. ; Stein, G. S. / A human histone (H2B.1 variant gene, located on chromosome 1, utilizes alternative 3' end processing. In: Journal of Cellular Biochemistry. 1992 ; Vol. 50, No. 4. pp. 374-385.
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