A human histone H2B.1 Variant gene, located on chromosome 1, utilizes alternative 3′ end processing

David Collart, Shirwin Pockwinse, Jane B. Lian, Janet L. Stein, Gary S. Stein, Paul L. Romain, Suzie Pilapil, Kay Heubner, Linda A. Cannizzaro, Carlo M. Croce

Research output: Contribution to journalArticle

25 Scopus citations

Abstract

Abstract a variant human H2B histone gene (GL 105), previously shown to encode a 2300 nt replication independent mRNA, has been cloned. We demonstrate this gene expresses alternative mRNA regulated differentially during the hela S3 cell cycle. The H2B‐GL105 gene encodes both a 500 nt cell cycle dependent mRNA and a 2300 nt constitutively expressed mRNA. The 3′ end of the cell cycle regulated mRNA terminates immediately following the region of hyphenated dyad symmetry typical of most histone mRNAs, whereas the constitutively expressed mRNA has a 1798 nt non‐translated trailer that contains the same region of hyphenated dyad symmetry but is polyadenylated. The cap site for the H2B‐GL105 mRNAs is located 42 nt upstream of the protein coding region. The H2B‐GL105 histone gene was localized to chromosome region 1q21‐1q23 by chromosomal in situ hybridization and by analysis of rodent‐human somatic cell hybrids using an H2B‐GL105 specific probe. The H2B‐GL105 gene is paired with a functional H2A histone gene and this H2B/H2B gene pair is seperated by a bidirectionally transcribed intergenic promoter region containing consensus TATA and CCAAT boxes and an OTF‐1 element. These results demonstrate that cell cycle regulated and constitutively expressed histone mRNAs can be encoded by the same gene, and indicate that alternative 3′ end processing may be an important mechanism for regulation of histone mRNA. Such control further increases the versatility by which cells can modulate the synthesis of replication‐dependent as well as variant histone proteins during the cell cycle and at the onset of differentiation. © 1992 Wiley‐Liss, Inc.

Original languageEnglish (US)
Pages (from-to)374-385
Number of pages12
JournalJournal of Cellular Biochemistry
Volume50
Issue number4
DOIs
StatePublished - Dec 1992

Keywords

  • alternative 3′processing
  • growth regulation
  • histone variant

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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    Collart, D., Pockwinse, S., Lian, J. B., Stein, J. L., Stein, G. S., Romain, P. L., Pilapil, S., Heubner, K., Cannizzaro, L. A., & Croce, C. M. (1992). A human histone H2B.1 Variant gene, located on chromosome 1, utilizes alternative 3′ end processing. Journal of Cellular Biochemistry, 50(4), 374-385. https://doi.org/10.1002/jcb.240500406