A high-throughput strategy for dissecting mammalian genetic interactions

Victoria B. Stockman, Lila Ghamsari, Gorka Lasso, Barry Honig, Sagi D. Shapira, Harris H. Wang

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

Comprehensive delineation of complex cellular networks requires high-throughput interrogation of genetic interactions. To address this challenge, we describe the development of a multiplex combinatorial strategy to assess pairwise genetic interactions using CRISPRCas9 genome editing and next-generation sequencing. We characterize the performance of combinatorial genome editing and analysis using different promoter and gRNA designs and identified regions of the chimeric RNA that are compatible with next-generation sequencing preparation and quantification. This approach is an important step towards elucidating genetic networks relevant to human diseases and the development of more efficient Cas9-based therapeutics.

Original languageEnglish (US)
Article numbere0167617
JournalPloS one
Volume11
Issue number12
DOIs
StatePublished - Dec 2016

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)
  • General

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