A high-throughput strategy for dissecting mammalian genetic interactions

Victoria B. Stockman, Lila Ghamsari, Gorka Lasso, Barry Honig, Sagi D. Shapira, Harris H. Wang

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Comprehensive delineation of complex cellular networks requires high-throughput interrogation of genetic interactions. To address this challenge, we describe the development of a multiplex combinatorial strategy to assess pairwise genetic interactions using CRISPRCas9 genome editing and next-generation sequencing. We characterize the performance of combinatorial genome editing and analysis using different promoter and gRNA designs and identified regions of the chimeric RNA that are compatible with next-generation sequencing preparation and quantification. This approach is an important step towards elucidating genetic networks relevant to human diseases and the development of more efficient Cas9-based therapeutics.

Original languageEnglish (US)
Article numbere0167617
JournalPloS one
Volume11
Issue number12
DOIs
StatePublished - Dec 2016
Externally publishedYes

Fingerprint

Guide RNA
Genes
Throughput
Human Development
genome
RNA
human diseases
promoter regions
therapeutics
Gene Editing
Therapeutics

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Stockman, V. B., Ghamsari, L., Lasso, G., Honig, B., Shapira, S. D., & Wang, H. H. (2016). A high-throughput strategy for dissecting mammalian genetic interactions. PloS one, 11(12), [e0167617]. https://doi.org/10.1371/journal.pone.0167617

A high-throughput strategy for dissecting mammalian genetic interactions. / Stockman, Victoria B.; Ghamsari, Lila; Lasso, Gorka; Honig, Barry; Shapira, Sagi D.; Wang, Harris H.

In: PloS one, Vol. 11, No. 12, e0167617, 12.2016.

Research output: Contribution to journalArticle

Stockman, VB, Ghamsari, L, Lasso, G, Honig, B, Shapira, SD & Wang, HH 2016, 'A high-throughput strategy for dissecting mammalian genetic interactions', PloS one, vol. 11, no. 12, e0167617. https://doi.org/10.1371/journal.pone.0167617
Stockman, Victoria B. ; Ghamsari, Lila ; Lasso, Gorka ; Honig, Barry ; Shapira, Sagi D. ; Wang, Harris H. / A high-throughput strategy for dissecting mammalian genetic interactions. In: PloS one. 2016 ; Vol. 11, No. 12.
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