A high-resolution radiation hybrid map of the human genome draft sequence

M. Olivier, A. Aggarwal, J. Allen, A. A. Almendras, E. S. Bajorek, E. M. Beasley, S. D. Brady, J. M. Bushard, V. I. Bustos, A. Chu, T. R. Chung, A. De Witte, M. E. Denys, R. Dominguez, N. Y. Fang, B. D. Foster, R. W. Freudenberg, D. Hadley, L. R. Hamilton, T. J. Jeffrey & 35 others Libusha Kelly, L. Lazzeroni, M. R. Levy, S. C. Lewis, X. Liu, F. J. Lopez, B. Louie, J. P. Marquis, R. A. Martinez, M. K. Matsuura, N. S. Misherghi, J. A. Norton, A. Olshen, S. M. Perkins, A. J. Perou, C. Piercy, M. Piercy, F. Qin, T. Reif, K. Sheppard, V. Shokoohi, G. A. Smick, W. L. Sun, E. A. Stewart, J. Fernando Tejeda, N. M. Tran, T. Trejo, N. T. Vo, S. C M Yan, D. L. Zierten, S. Zhao, R. Sachidanandam, B. J. Trask, R. M. Myers, D. R. Cox

Research output: Contribution to journalArticle

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Abstract

We have constructed a physical map of the human genome by using a panel of 90 whole-genome radiation hybrids (the TNG panel) in conjunction with 40,322 sequence-tagged sites (STSs) derived from random genomic sequences as well as expressed sequences. Of 36,678 STSs on the TNG radiation hybrid map, only 3604 (9.8%) were absent from the unassembled draft sequence of the human genome. Of 20,030 STSs ordered on the TNG map as well as the assembled human genome draft sequence and the Celera assembled human genome sequence, 36% of the STSs had a discrepant order between the working draft sequence and the Celera sequence. The TNG map order was identical to one of the two sequence orders in 60% of these discrepant cases.

Original languageEnglish (US)
Pages (from-to)1298-1302
Number of pages5
JournalScience
Volume291
Issue number5507
DOIs
StatePublished - Feb 16 2001
Externally publishedYes

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Sequence Tagged Sites
Radiation Hybrid Mapping
Human Genome
Genome

ASJC Scopus subject areas

  • General

Cite this

Olivier, M., Aggarwal, A., Allen, J., Almendras, A. A., Bajorek, E. S., Beasley, E. M., ... Cox, D. R. (2001). A high-resolution radiation hybrid map of the human genome draft sequence. Science, 291(5507), 1298-1302. https://doi.org/10.1126/science.1057437

A high-resolution radiation hybrid map of the human genome draft sequence. / Olivier, M.; Aggarwal, A.; Allen, J.; Almendras, A. A.; Bajorek, E. S.; Beasley, E. M.; Brady, S. D.; Bushard, J. M.; Bustos, V. I.; Chu, A.; Chung, T. R.; De Witte, A.; Denys, M. E.; Dominguez, R.; Fang, N. Y.; Foster, B. D.; Freudenberg, R. W.; Hadley, D.; Hamilton, L. R.; Jeffrey, T. J.; Kelly, Libusha; Lazzeroni, L.; Levy, M. R.; Lewis, S. C.; Liu, X.; Lopez, F. J.; Louie, B.; Marquis, J. P.; Martinez, R. A.; Matsuura, M. K.; Misherghi, N. S.; Norton, J. A.; Olshen, A.; Perkins, S. M.; Perou, A. J.; Piercy, C.; Piercy, M.; Qin, F.; Reif, T.; Sheppard, K.; Shokoohi, V.; Smick, G. A.; Sun, W. L.; Stewart, E. A.; Fernando Tejeda, J.; Tran, N. M.; Trejo, T.; Vo, N. T.; Yan, S. C M; Zierten, D. L.; Zhao, S.; Sachidanandam, R.; Trask, B. J.; Myers, R. M.; Cox, D. R.

In: Science, Vol. 291, No. 5507, 16.02.2001, p. 1298-1302.

Research output: Contribution to journalArticle

Olivier, M, Aggarwal, A, Allen, J, Almendras, AA, Bajorek, ES, Beasley, EM, Brady, SD, Bushard, JM, Bustos, VI, Chu, A, Chung, TR, De Witte, A, Denys, ME, Dominguez, R, Fang, NY, Foster, BD, Freudenberg, RW, Hadley, D, Hamilton, LR, Jeffrey, TJ, Kelly, L, Lazzeroni, L, Levy, MR, Lewis, SC, Liu, X, Lopez, FJ, Louie, B, Marquis, JP, Martinez, RA, Matsuura, MK, Misherghi, NS, Norton, JA, Olshen, A, Perkins, SM, Perou, AJ, Piercy, C, Piercy, M, Qin, F, Reif, T, Sheppard, K, Shokoohi, V, Smick, GA, Sun, WL, Stewart, EA, Fernando Tejeda, J, Tran, NM, Trejo, T, Vo, NT, Yan, SCM, Zierten, DL, Zhao, S, Sachidanandam, R, Trask, BJ, Myers, RM & Cox, DR 2001, 'A high-resolution radiation hybrid map of the human genome draft sequence', Science, vol. 291, no. 5507, pp. 1298-1302. https://doi.org/10.1126/science.1057437
Olivier M, Aggarwal A, Allen J, Almendras AA, Bajorek ES, Beasley EM et al. A high-resolution radiation hybrid map of the human genome draft sequence. Science. 2001 Feb 16;291(5507):1298-1302. https://doi.org/10.1126/science.1057437
Olivier, M. ; Aggarwal, A. ; Allen, J. ; Almendras, A. A. ; Bajorek, E. S. ; Beasley, E. M. ; Brady, S. D. ; Bushard, J. M. ; Bustos, V. I. ; Chu, A. ; Chung, T. R. ; De Witte, A. ; Denys, M. E. ; Dominguez, R. ; Fang, N. Y. ; Foster, B. D. ; Freudenberg, R. W. ; Hadley, D. ; Hamilton, L. R. ; Jeffrey, T. J. ; Kelly, Libusha ; Lazzeroni, L. ; Levy, M. R. ; Lewis, S. C. ; Liu, X. ; Lopez, F. J. ; Louie, B. ; Marquis, J. P. ; Martinez, R. A. ; Matsuura, M. K. ; Misherghi, N. S. ; Norton, J. A. ; Olshen, A. ; Perkins, S. M. ; Perou, A. J. ; Piercy, C. ; Piercy, M. ; Qin, F. ; Reif, T. ; Sheppard, K. ; Shokoohi, V. ; Smick, G. A. ; Sun, W. L. ; Stewart, E. A. ; Fernando Tejeda, J. ; Tran, N. M. ; Trejo, T. ; Vo, N. T. ; Yan, S. C M ; Zierten, D. L. ; Zhao, S. ; Sachidanandam, R. ; Trask, B. J. ; Myers, R. M. ; Cox, D. R. / A high-resolution radiation hybrid map of the human genome draft sequence. In: Science. 2001 ; Vol. 291, No. 5507. pp. 1298-1302.
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T1 - A high-resolution radiation hybrid map of the human genome draft sequence

AU - Olivier, M.

AU - Aggarwal, A.

AU - Allen, J.

AU - Almendras, A. A.

AU - Bajorek, E. S.

AU - Beasley, E. M.

AU - Brady, S. D.

AU - Bushard, J. M.

AU - Bustos, V. I.

AU - Chu, A.

AU - Chung, T. R.

AU - De Witte, A.

AU - Denys, M. E.

AU - Dominguez, R.

AU - Fang, N. Y.

AU - Foster, B. D.

AU - Freudenberg, R. W.

AU - Hadley, D.

AU - Hamilton, L. R.

AU - Jeffrey, T. J.

AU - Kelly, Libusha

AU - Lazzeroni, L.

AU - Levy, M. R.

AU - Lewis, S. C.

AU - Liu, X.

AU - Lopez, F. J.

AU - Louie, B.

AU - Marquis, J. P.

AU - Martinez, R. A.

AU - Matsuura, M. K.

AU - Misherghi, N. S.

AU - Norton, J. A.

AU - Olshen, A.

AU - Perkins, S. M.

AU - Perou, A. J.

AU - Piercy, C.

AU - Piercy, M.

AU - Qin, F.

AU - Reif, T.

AU - Sheppard, K.

AU - Shokoohi, V.

AU - Smick, G. A.

AU - Sun, W. L.

AU - Stewart, E. A.

AU - Fernando Tejeda, J.

AU - Tran, N. M.

AU - Trejo, T.

AU - Vo, N. T.

AU - Yan, S. C M

AU - Zierten, D. L.

AU - Zhao, S.

AU - Sachidanandam, R.

AU - Trask, B. J.

AU - Myers, R. M.

AU - Cox, D. R.

PY - 2001/2/16

Y1 - 2001/2/16

N2 - We have constructed a physical map of the human genome by using a panel of 90 whole-genome radiation hybrids (the TNG panel) in conjunction with 40,322 sequence-tagged sites (STSs) derived from random genomic sequences as well as expressed sequences. Of 36,678 STSs on the TNG radiation hybrid map, only 3604 (9.8%) were absent from the unassembled draft sequence of the human genome. Of 20,030 STSs ordered on the TNG map as well as the assembled human genome draft sequence and the Celera assembled human genome sequence, 36% of the STSs had a discrepant order between the working draft sequence and the Celera sequence. The TNG map order was identical to one of the two sequence orders in 60% of these discrepant cases.

AB - We have constructed a physical map of the human genome by using a panel of 90 whole-genome radiation hybrids (the TNG panel) in conjunction with 40,322 sequence-tagged sites (STSs) derived from random genomic sequences as well as expressed sequences. Of 36,678 STSs on the TNG radiation hybrid map, only 3604 (9.8%) were absent from the unassembled draft sequence of the human genome. Of 20,030 STSs ordered on the TNG map as well as the assembled human genome draft sequence and the Celera assembled human genome sequence, 36% of the STSs had a discrepant order between the working draft sequence and the Celera sequence. The TNG map order was identical to one of the two sequence orders in 60% of these discrepant cases.

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