A heteromorphic protein-tyrosine phosphatase, PTPφ, is regulated by CSF-1 in macrophages

Fiona J. Pixley, Pierre S W Lee, Melissa G. Dominguez, Douglas B. Einstein, E. Richard Stanley

Research output: Contribution to journalArticle

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Abstract

A novel protein-tyrosine phosphatase, PTPφ, was cloned from a murine macrophage cDNA library. As a result of alternative splicing, macrophage PTPφ mRNAs are predicted to encode two membrane-spanning molecules and a cytosolic enzyme with identical catalytic domains. The membrane-spanning forms differ in the juxtamembrane region, while a start codon downstream of this region is utilized in the translation of the putative cytosolic form. Expression of PTPφ mRNA is low and restricted to macrophage cell lines, macrophage-rich tissues, and brain, kidney, and heart. The mRNA in macrophages and heart is ∼2.8 kilobases (kb). However, a ∼5.5-kb transcript in brain and kidney indicates a fourth isoform encoding a large extracellular domain. The ∼5.5-kb PTPφ brain mRNA encodes the mouse homolog of GLEPP1, a recently reported glomerular epithelial protein. The level of expression of the mRNA encoding the cytosolic form was very low, and only the membrane-spanning proteins (43 and 47 kDa) could be detected in macrophages. Following addition of colony stimulating factor-1 to quiescent BAC1.2F5 macrophages, PTPφ mRNA and protein were down-regulated. The restricted expression of the shorter isoforms of PTPφ and their regulation by colony stimulating factor-1 in macrophages suggest that PTPφ may play a role in mononuclear phagocyte survival, proliferation, and/or differentiation.

Original languageEnglish (US)
Pages (from-to)27339-27347
Number of pages9
JournalJournal of Biological Chemistry
Volume270
Issue number45
StatePublished - Nov 10 1995

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Protein Tyrosine Phosphatases
Macrophage Colony-Stimulating Factor
Macrophages
Messenger RNA
Brain
Membranes
Protein Isoforms
Kidney
Proteins
Initiator Codon
Alternative Splicing
Phagocytes
Gene Library
Catalytic Domain
Membrane Proteins
Cells
Tissue
Cell Line
Molecules
Enzymes

ASJC Scopus subject areas

  • Biochemistry

Cite this

Pixley, F. J., Lee, P. S. W., Dominguez, M. G., Einstein, D. B., & Stanley, E. R. (1995). A heteromorphic protein-tyrosine phosphatase, PTPφ, is regulated by CSF-1 in macrophages. Journal of Biological Chemistry, 270(45), 27339-27347.

A heteromorphic protein-tyrosine phosphatase, PTPφ, is regulated by CSF-1 in macrophages. / Pixley, Fiona J.; Lee, Pierre S W; Dominguez, Melissa G.; Einstein, Douglas B.; Stanley, E. Richard.

In: Journal of Biological Chemistry, Vol. 270, No. 45, 10.11.1995, p. 27339-27347.

Research output: Contribution to journalArticle

Pixley, FJ, Lee, PSW, Dominguez, MG, Einstein, DB & Stanley, ER 1995, 'A heteromorphic protein-tyrosine phosphatase, PTPφ, is regulated by CSF-1 in macrophages', Journal of Biological Chemistry, vol. 270, no. 45, pp. 27339-27347.
Pixley FJ, Lee PSW, Dominguez MG, Einstein DB, Stanley ER. A heteromorphic protein-tyrosine phosphatase, PTPφ, is regulated by CSF-1 in macrophages. Journal of Biological Chemistry. 1995 Nov 10;270(45):27339-27347.
Pixley, Fiona J. ; Lee, Pierre S W ; Dominguez, Melissa G. ; Einstein, Douglas B. ; Stanley, E. Richard. / A heteromorphic protein-tyrosine phosphatase, PTPφ, is regulated by CSF-1 in macrophages. In: Journal of Biological Chemistry. 1995 ; Vol. 270, No. 45. pp. 27339-27347.
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