A general RNA motif for cellular transfection

Maria L.B. Magalhães, Michelle Byrom, Amy Yan, Linsley Kelly, Na Li, Raquel Furtado, Deborah Palliser, Andrew D. Ellington, Matthew Levy

Research output: Contribution to journalArticle

20 Scopus citations

Abstract

We have developed a selection scheme to generate nucleic acid sequences that recognize and directly internalize into mammalian cells without the aid of conventional delivery methods. To demonstrate the generality of the technology, two independent selections with different starting pools were performed against distinct target cells. Each selection yielded a single highly functional sequence, both of which folded into a common core structure. This internalization signal can be adapted for use as a general purpose reagent for transfection into a wide variety of cell types including primary cells.

Original languageEnglish (US)
Pages (from-to)616-624
Number of pages9
JournalMolecular Therapy
Volume20
Issue number3
DOIs
Publication statusPublished - Mar 2012

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ASJC Scopus subject areas

  • Molecular Medicine
  • Molecular Biology
  • Genetics
  • Pharmacology
  • Drug Discovery

Cite this

Magalhães, M. L. B., Byrom, M., Yan, A., Kelly, L., Li, N., Furtado, R., ... Levy, M. (2012). A general RNA motif for cellular transfection. Molecular Therapy, 20(3), 616-624. https://doi.org/10.1038/mt.2011.277