A fusion protein required for vesicle-mediated transport in both mammalian cells and yeast

Duncan W. Wilson, Celeste A. Wilcox, Gregory C. Flynn, Ellson Chen, Wun Jing Kuang, William J. Henzel, Marc R. Block, Axel Ullrich, James E. Rothman

Research output: Contribution to journalArticle

353 Scopus citations

Abstract

A protein sensitive toN-ethylmaleimide catalyses the fusion of transport vesicles with Golgi cisternae in a mammalian cell-free system. By cloning and sequencing its gene from Chinese hamster ovary cells and by use of in vitro assays, we show that this fusion protein is equivalent to the SEC18 gene product of the yeast Saccharomyces cerevisiae,known to be essential for vesicle-mediated transport from the endoplasmic reticulum to the Golgi apparatus. The mechanism of vesicular fusion is thus highly conserved, both between species and at different stages of transport.

Original languageEnglish (US)
Pages (from-to)355-359
Number of pages5
JournalNature
Volume339
Issue number6223
DOIs
Publication statusPublished - Jan 1 1989
Externally publishedYes

    Fingerprint

ASJC Scopus subject areas

  • General

Cite this

Wilson, D. W., Wilcox, C. A., Flynn, G. C., Chen, E., Kuang, W. J., Henzel, W. J., ... Rothman, J. E. (1989). A fusion protein required for vesicle-mediated transport in both mammalian cells and yeast. Nature, 339(6223), 355-359. https://doi.org/10.1038/339355a0