A functional in vivo screen for regulators of tumor progression identifies HOXB2 as a regulator of tumor growth in breast cancer

Pamela J. Boimel, Cristian Cruz, Jeffrey E. Segall

Research output: Contribution to journalArticle

16 Scopus citations

Abstract

Microarray profiling in breast cancer patients has identified genes correlated with prognosis whose functions are unknown. The purpose of this study was to develop an in vivo assay for functionally screening regulators of tumor progression using a mouse model. Transductant shRNA cell lines were made in the MDA-MB-231 breast cancer line. A pooled population of 25 transductants was injected into the mammary fat pads and tail veins of mice to evaluate tumor growth, and experimental metastasis. The proportions of transductants were evaluated in the tumor and metastases using barcodes specific to each shRNA transductant. We characterized the homeobox 2 transcription factor as a negative regulator, decreasing tumor growth in MDA-MB-231, T47D, and MTLn3 mammary adenocarcinoma cell lines. Homeobox genes have been correlated with cancer patient prognosis and tumorigenesis. Here we use a novel in vivo shRNA screen to identify a new role for a homeobox gene in human mammary adenocarcinoma.

Original languageEnglish (US)
Pages (from-to)164-172
Number of pages9
JournalGenomics
Volume98
Issue number3
DOIs
StatePublished - Sep 1 2011

Keywords

  • Breast cancer
  • Functional screen
  • Homeobox 2
  • ShRNA

ASJC Scopus subject areas

  • Genetics

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