@inbook{c3a48045203841b1a1b5446f6a617109,
title = "A fluorescence-based assay for identification of bacterial topoisomerase I poisons",
abstract = "Bacterial Topoisomerase I is a potential target for the identification of novel topoisomerase poison inhibitors that could provide leads for a new class of antibacterial compounds. Here we describe in detail a fluorescence-based cleavage assay that is successfully used in HTS for the discovery of bacterial topoisomerase Ι poisons.",
keywords = "Bacterial topoisomerase I, Fluorescence-based assay, High-throughput screening, Topoisomerase I poisons",
author = "Thirunavukkarasu Annamalai and Bokun Cheng and Neelam Keswani and Tse-Dinh, {Yuk Ching}",
note = "Funding Information: We thank the staff at NERCE/NSRB for access to screening libraries and HTS facilities, assistance in assay setup, data processing and analysis. This work was supported by funding from TB Alliance and National Institutes of Health grants R21NS067592, R01AI069313 to YT. Publisher Copyright: {\textcopyright} 2018, Springer Science+Business Media, LLC. Copyright: Copyright 2018 Elsevier B.V., All rights reserved.",
year = "2018",
doi = "10.1007/978-1-4939-7459-7_18",
language = "English (US)",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "259--268",
booktitle = "Methods in Molecular Biology",
}