Abstract
Bacterial Topoisomerase I is a potential target for the identification of novel topoisomerase poison inhibitors that could provide leads for a new class of antibacterial compounds. Here we describe in detail a fluorescence-based cleavage assay that is successfully used in HTS for the discovery of bacterial topoisomerase Ι poisons.
| Original language | English (US) |
|---|---|
| Title of host publication | Methods in Molecular Biology |
| Publisher | Humana Press Inc. |
| Pages | 259-268 |
| Number of pages | 10 |
| DOIs | |
| State | Published - Jan 1 2018 |
Publication series
| Name | Methods in Molecular Biology |
|---|---|
| Volume | 1703 |
| ISSN (Print) | 1064-3745 |
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Keywords
- Bacterial topoisomerase I
- Fluorescence-based assay
- High-throughput screening
- Topoisomerase I poisons
ASJC Scopus subject areas
- Molecular Biology
- Genetics
Cite this
A fluorescence-based assay for identification of bacterial topoisomerase I poisons. / Annamalai, Thirunavukkarasu; Cheng, Bokun; Keswani, Neelam; Tse-Dinh, Yuk Ching.
Methods in Molecular Biology. Humana Press Inc., 2018. p. 259-268 (Methods in Molecular Biology; Vol. 1703).Research output: Chapter in Book/Report/Conference proceeding › Chapter
}
TY - CHAP
T1 - A fluorescence-based assay for identification of bacterial topoisomerase I poisons
AU - Annamalai, Thirunavukkarasu
AU - Cheng, Bokun
AU - Keswani, Neelam
AU - Tse-Dinh, Yuk Ching
PY - 2018/1/1
Y1 - 2018/1/1
N2 - Bacterial Topoisomerase I is a potential target for the identification of novel topoisomerase poison inhibitors that could provide leads for a new class of antibacterial compounds. Here we describe in detail a fluorescence-based cleavage assay that is successfully used in HTS for the discovery of bacterial topoisomerase Ι poisons.
AB - Bacterial Topoisomerase I is a potential target for the identification of novel topoisomerase poison inhibitors that could provide leads for a new class of antibacterial compounds. Here we describe in detail a fluorescence-based cleavage assay that is successfully used in HTS for the discovery of bacterial topoisomerase Ι poisons.
KW - Bacterial topoisomerase I
KW - Fluorescence-based assay
KW - High-throughput screening
KW - Topoisomerase I poisons
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UR - http://www.scopus.com/inward/citedby.url?scp=85035104311&partnerID=8YFLogxK
U2 - 10.1007/978-1-4939-7459-7_18
DO - 10.1007/978-1-4939-7459-7_18
M3 - Chapter
C2 - 29177747
AN - SCOPUS:85035104311
T3 - Methods in Molecular Biology
SP - 259
EP - 268
BT - Methods in Molecular Biology
PB - Humana Press Inc.
ER -