A fluorescence-based assay for identification of bacterial topoisomerase I poisons

Thirunavukkarasu Annamalai, Bokun Cheng, Neelam Keswani, Yuk Ching Tse-Dinh

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Bacterial Topoisomerase I is a potential target for the identification of novel topoisomerase poison inhibitors that could provide leads for a new class of antibacterial compounds. Here we describe in detail a fluorescence-based cleavage assay that is successfully used in HTS for the discovery of bacterial topoisomerase Ι poisons.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages259-268
Number of pages10
DOIs
StatePublished - Jan 1 2018

Publication series

NameMethods in Molecular Biology
Volume1703
ISSN (Print)1064-3745

Fingerprint

Type I DNA Topoisomerase
Poisons
Fluorescence
Topoisomerase Inhibitors

Keywords

  • Bacterial topoisomerase I
  • Fluorescence-based assay
  • High-throughput screening
  • Topoisomerase I poisons

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Annamalai, T., Cheng, B., Keswani, N., & Tse-Dinh, Y. C. (2018). A fluorescence-based assay for identification of bacterial topoisomerase I poisons. In Methods in Molecular Biology (pp. 259-268). (Methods in Molecular Biology; Vol. 1703). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-7459-7_18

A fluorescence-based assay for identification of bacterial topoisomerase I poisons. / Annamalai, Thirunavukkarasu; Cheng, Bokun; Keswani, Neelam; Tse-Dinh, Yuk Ching.

Methods in Molecular Biology. Humana Press Inc., 2018. p. 259-268 (Methods in Molecular Biology; Vol. 1703).

Research output: Chapter in Book/Report/Conference proceedingChapter

Annamalai, T, Cheng, B, Keswani, N & Tse-Dinh, YC 2018, A fluorescence-based assay for identification of bacterial topoisomerase I poisons. in Methods in Molecular Biology. Methods in Molecular Biology, vol. 1703, Humana Press Inc., pp. 259-268. https://doi.org/10.1007/978-1-4939-7459-7_18
Annamalai T, Cheng B, Keswani N, Tse-Dinh YC. A fluorescence-based assay for identification of bacterial topoisomerase I poisons. In Methods in Molecular Biology. Humana Press Inc. 2018. p. 259-268. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-4939-7459-7_18
Annamalai, Thirunavukkarasu ; Cheng, Bokun ; Keswani, Neelam ; Tse-Dinh, Yuk Ching. / A fluorescence-based assay for identification of bacterial topoisomerase I poisons. Methods in Molecular Biology. Humana Press Inc., 2018. pp. 259-268 (Methods in Molecular Biology).
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