A fluorescence-based assay for identification of bacterial topoisomerase I poisons

Thirunavukkarasu Annamalai, Bokun Cheng, Neelam Keswani, Yuk Ching Tse-Dinh

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Bacterial Topoisomerase I is a potential target for the identification of novel topoisomerase poison inhibitors that could provide leads for a new class of antibacterial compounds. Here we describe in detail a fluorescence-based cleavage assay that is successfully used in HTS for the discovery of bacterial topoisomerase Ι poisons.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages259-268
Number of pages10
DOIs
Publication statusPublished - Jan 1 2018

Publication series

NameMethods in Molecular Biology
Volume1703
ISSN (Print)1064-3745

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Keywords

  • Bacterial topoisomerase I
  • Fluorescence-based assay
  • High-throughput screening
  • Topoisomerase I poisons

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Annamalai, T., Cheng, B., Keswani, N., & Tse-Dinh, Y. C. (2018). A fluorescence-based assay for identification of bacterial topoisomerase I poisons. In Methods in Molecular Biology (pp. 259-268). (Methods in Molecular Biology; Vol. 1703). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-7459-7_18