A fluorescence-based assay for identification of bacterial topoisomerase I poisons

Thirunavukkarasu Annamalai; Bokun Cheng; Neelam Keswani; Yuk Ching Tse-Dinh

doi:10.1007/978-1-4939-7459-7_18

A fluorescence-based assay for identification of bacterial topoisomerase I poisons

Thirunavukkarasu Annamalai, Bokun Cheng, Neelam Keswani, Yuk Ching Tse-Dinh

Pediatrics

Research output: Chapter in Book/Report/Conference proceeding › Chapter

1 Scopus citations

Abstract

Bacterial Topoisomerase I is a potential target for the identification of novel topoisomerase poison inhibitors that could provide leads for a new class of antibacterial compounds. Here we describe in detail a fluorescence-based cleavage assay that is successfully used in HTS for the discovery of bacterial topoisomerase Ι poisons.

Original language	English (US)
Title of host publication	Methods in Molecular Biology
Publisher	Humana Press Inc.
Pages	259-268
Number of pages	10
DOIs	https://doi.org/10.1007/978-1-4939-7459-7_18
State	Published - Jan 1 2018

Publication series

Name	Methods in Molecular Biology
Volume	1703
ISSN (Print)	1064-3745

Keywords

Bacterial topoisomerase I
Fluorescence-based assay
High-throughput screening
Topoisomerase I poisons

ASJC Scopus subject areas

Molecular Biology
Genetics

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10.1007/978-1-4939-7459-7_18

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Annamalai, T., Cheng, B., Keswani, N., & Tse-Dinh, Y. C. (2018). A fluorescence-based assay for identification of bacterial topoisomerase I poisons. In Methods in Molecular Biology (pp. 259-268). (Methods in Molecular Biology; Vol. 1703). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-7459-7_18

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