A family of moderately repetitive sequences in mouse DNA

Sheau Mei Cheng, Carl L. Schildkraut

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

When mouse DNA is digested to completion with restriction endonuclease Eco R1, a distinct band of 1.3 kb segments comprising about 0.5-3% of the genome is observed upon agarose gel electrophoresis. This DNA is not tandemly repeated in the genome and is not derived from mouse satellite DNA. Restriction endonuclease analysis suggested that the 1.3 kb segments are heterogeneous. Specific sequences were selected from the 1.3 kb segments and amplified by cloning in plasmid pBR322. Southern transfer experiments Indicated that three separately cloned mouse DNA inserts hybridized predominantly to the Eco R1 1.3 kb band and to the conspicuous subsegments generated by secondary restriction endonuclease cleavage of the sucrose gradient purified 1.3 kb segments. Segments were also excised by Hha I (Hha I segments) from the chimeric plasmids containing mouse DNA inserts and subjected to restriction endonuclease and cross-hybridization analysis. It was found that the three Hha I segments were different, although two of them exhibited partial sequence homology. Cot analysis indicated that each of the Hha I segments are repeated about 104 times in the mouse genome. These findings indicate that a family of related but non-identical, moderately repetitive DNA sequences, rather than a single homogeneous repeat, is present in the 1.3 kb Eco R1 band.

Original languageEnglish (US)
Pages (from-to)4075-4090
Number of pages16
JournalNucleic Acids Research
Volume8
Issue number18
DOIs
StatePublished - Sep 25 1980

Fingerprint

Nucleic Acid Repetitive Sequences
DNA Restriction Enzymes
Mouse
DNA
Genes
Genome
Plasmids
Restriction
Satellite DNA
Cloning
DNA sequences
Electrophoresis
Sepharose
Agar Gel Electrophoresis
Sucrose
Sugar (sucrose)
Sequence Homology
Gels
Organism Cloning
Family

ASJC Scopus subject areas

  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Health, Toxicology and Mutagenesis
  • Toxicology
  • Genetics(clinical)
  • Genetics

Cite this

A family of moderately repetitive sequences in mouse DNA. / Cheng, Sheau Mei; Schildkraut, Carl L.

In: Nucleic Acids Research, Vol. 8, No. 18, 25.09.1980, p. 4075-4090.

Research output: Contribution to journalArticle

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