A factor produced by cultured rat leydig tumor (rice-500) cells associated with humoral hypercalcemia stimulates adenosine 3′, 5′-monophosphate production via the parathyroid hormone receptor in human skin fibroblasts

Caroline Silve, Arthur Santora, Allen Spiegel

Research output: Contribution to journalArticle

23 Scopus citations


The Rice-500 Leydig cell tumor of Fischer rats is associated with humoral hypercalcemia invivo and produces a factor that stimulates cAMP formation in cultured rat osteosarcoma cells. We found that cultured human skin fibroblasts respond to both human PTH-(1 34) and the factor produced by cultured rat Leydig tumor cells with a dose-dependent rise in cAMP formation. The time courses for stimulation of the two agents were similar, and stimulation by both was blocked by the competitive PTH antagonist [8, 18-norleucine, 34-tyrosine]bovine PTH-(3 34) amide. These data suggest that PTH-like factors secreted by a murine tumor are capable of interacting with the human PTH receptor.

Original languageEnglish (US)
Pages (from-to)1144-1147
Number of pages4
JournalJournal of Clinical Endocrinology and Metabolism
Issue number6
StatePublished - Jun 1985


ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Biochemistry
  • Endocrinology
  • Clinical Biochemistry
  • Biochemistry, medical

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