A Drosophila kinesin required for synaptic bouton formation and synaptic vesicle transport

Eunju Pack-Chung, Peri T. Kurshan, Dion K. Dickman, Thomas L. Schwarz

Research output: Contribution to journalArticle

109 Citations (Scopus)

Abstract

The morphological transition of growth cones to synaptic boutons characterizes synaptogenesis. Here we have isolated mutations in immaculate connections (imac; CG8566), a previously uncharacterized Drosophila gene encoding a member of the Kinesin-3 family. Whereas earlier studies in Drosophila implicated Kinesin-1 in transporting synaptic vesicle precursors, we find that Imac is essential for this transport. An unexpected feature of imac mutants is the failure of synaptic boutons to form. Motor neurons lacking imac properly target to muscles but remain within target fields as thin processes, a structure that is distinct from either growth cones or mature terminals. Few active zones form at these endings. We show that the arrest of synaptogenesis is not a secondary consequence of the absence of transmission. Our data thus indicate that Imac transports components required for synaptic maturation and provide insight into presynaptic maturation as a process that can be differentiated from axon outgrowth and targeting.

Original languageEnglish (US)
Pages (from-to)980-989
Number of pages10
JournalNature Neuroscience
Volume10
Issue number8
DOIs
StatePublished - Aug 1 2007
Externally publishedYes

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Kinesin
Growth Cones
Synaptic Vesicles
Presynaptic Terminals
Drosophila
Motor Neurons
Muscles
Mutation
Genes
Neuronal Outgrowth

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

A Drosophila kinesin required for synaptic bouton formation and synaptic vesicle transport. / Pack-Chung, Eunju; Kurshan, Peri T.; Dickman, Dion K.; Schwarz, Thomas L.

In: Nature Neuroscience, Vol. 10, No. 8, 01.08.2007, p. 980-989.

Research output: Contribution to journalArticle

Pack-Chung, Eunju ; Kurshan, Peri T. ; Dickman, Dion K. ; Schwarz, Thomas L. / A Drosophila kinesin required for synaptic bouton formation and synaptic vesicle transport. In: Nature Neuroscience. 2007 ; Vol. 10, No. 8. pp. 980-989.
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