A dominant positive and negative selectable gene for use in mammalian cells

Faina Schwartz, Nobuyo Maeda, Oliver Smithies, Robert Hickey, Winfried Edelmann, Arthur I. Skoultchi, Raju Kucherlapati

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

We have constructed three different fusion genes containing the herpes simplex virus thymidine kinase (HSV tk) and the bacterial neomycin phosphotransferase (neo) genes. All three fusion genes utilize the HSV tk promoter but differ at the junction of their components. We have determined if the fusion genes are bifunctional by introducing them into mammalian cells and testing for function of the individual components. One of the fusion genes, TNFUS 69, produced a bicistronic message and a fusion protein that has TK and NEO protein functions. This and other fusion genes of a similar nature could serve as dominant positive and negative selectable markers in mammalian cells. (.

Original languageEnglish (US)
Pages (from-to)10416-10420
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume88
Issue number23
StatePublished - 1991

Fingerprint

Gene Fusion
Thymidine Kinase
Genes
Simplexvirus
Kanamycin Kinase
Proteins

Keywords

  • Bacterial neomycin phosphotransferase
  • Gene fusionh
  • Herpes simplex virus thymidine kinase

ASJC Scopus subject areas

  • General
  • Genetics

Cite this

A dominant positive and negative selectable gene for use in mammalian cells. / Schwartz, Faina; Maeda, Nobuyo; Smithies, Oliver; Hickey, Robert; Edelmann, Winfried; Skoultchi, Arthur I.; Kucherlapati, Raju.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 88, No. 23, 1991, p. 10416-10420.

Research output: Contribution to journalArticle

Schwartz, Faina ; Maeda, Nobuyo ; Smithies, Oliver ; Hickey, Robert ; Edelmann, Winfried ; Skoultchi, Arthur I. ; Kucherlapati, Raju. / A dominant positive and negative selectable gene for use in mammalian cells. In: Proceedings of the National Academy of Sciences of the United States of America. 1991 ; Vol. 88, No. 23. pp. 10416-10420.
@article{54460f7dbe2e42bcaa0d67b08445471a,
title = "A dominant positive and negative selectable gene for use in mammalian cells",
abstract = "We have constructed three different fusion genes containing the herpes simplex virus thymidine kinase (HSV tk) and the bacterial neomycin phosphotransferase (neo) genes. All three fusion genes utilize the HSV tk promoter but differ at the junction of their components. We have determined if the fusion genes are bifunctional by introducing them into mammalian cells and testing for function of the individual components. One of the fusion genes, TNFUS 69, produced a bicistronic message and a fusion protein that has TK and NEO protein functions. This and other fusion genes of a similar nature could serve as dominant positive and negative selectable markers in mammalian cells. (.",
keywords = "Bacterial neomycin phosphotransferase, Gene fusionh, Herpes simplex virus thymidine kinase",
author = "Faina Schwartz and Nobuyo Maeda and Oliver Smithies and Robert Hickey and Winfried Edelmann and Skoultchi, {Arthur I.} and Raju Kucherlapati",
year = "1991",
language = "English (US)",
volume = "88",
pages = "10416--10420",
journal = "Proceedings of the National Academy of Sciences of the United States of America",
issn = "0027-8424",
number = "23",

}

TY - JOUR

T1 - A dominant positive and negative selectable gene for use in mammalian cells

AU - Schwartz, Faina

AU - Maeda, Nobuyo

AU - Smithies, Oliver

AU - Hickey, Robert

AU - Edelmann, Winfried

AU - Skoultchi, Arthur I.

AU - Kucherlapati, Raju

PY - 1991

Y1 - 1991

N2 - We have constructed three different fusion genes containing the herpes simplex virus thymidine kinase (HSV tk) and the bacterial neomycin phosphotransferase (neo) genes. All three fusion genes utilize the HSV tk promoter but differ at the junction of their components. We have determined if the fusion genes are bifunctional by introducing them into mammalian cells and testing for function of the individual components. One of the fusion genes, TNFUS 69, produced a bicistronic message and a fusion protein that has TK and NEO protein functions. This and other fusion genes of a similar nature could serve as dominant positive and negative selectable markers in mammalian cells. (.

AB - We have constructed three different fusion genes containing the herpes simplex virus thymidine kinase (HSV tk) and the bacterial neomycin phosphotransferase (neo) genes. All three fusion genes utilize the HSV tk promoter but differ at the junction of their components. We have determined if the fusion genes are bifunctional by introducing them into mammalian cells and testing for function of the individual components. One of the fusion genes, TNFUS 69, produced a bicistronic message and a fusion protein that has TK and NEO protein functions. This and other fusion genes of a similar nature could serve as dominant positive and negative selectable markers in mammalian cells. (.

KW - Bacterial neomycin phosphotransferase

KW - Gene fusionh

KW - Herpes simplex virus thymidine kinase

UR - http://www.scopus.com/inward/record.url?scp=0025719001&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025719001&partnerID=8YFLogxK

M3 - Article

C2 - 1720540

AN - SCOPUS:0025719001

VL - 88

SP - 10416

EP - 10420

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

SN - 0027-8424

IS - 23

ER -