A Dictyostelium mutant lacking an F-actin cross-linking protein, the 120-kD gelation factor

Marijke Brink, Günther Gerisch, Gerhard Isenberg, Angelika A. Noegel, Jeffrey E. Segall, Eva Wallraff, Michael Schleicher

Research output: Contribution to journalArticle

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Abstract

Actin-binding proteins are known to regulate in vitro the assembly of actin into supramolecular structures, but evidence for their activities in living nonmuscle cells is scarce. Amebae of Dictyostelium discoideum are nonmuscle cells in which mutants defective in several actin-binding proteins have been described. Here we characterize a mutant deficient in the 120-kD gelation factor, one of the most abundant F-actin cross-linking proteins of D. discoideum cells. No F-actin cross-linking activity attributable to the 120-kD protein was detected in mutant cell extracts, and antibodies recognizing different epitopes on the polypeptide showed the entire protein was lacking. Under the conditions used, elimination of the gelation factor did not substantially alter growth, shape, motility, or chemotactic orientation of the cells towards a cAMP source. Aggregates of the mutant developed into fruiting bodies consisting of normally differentiated spores and stalk cells. In cytoskeleton preparations a dense network of actin filaments as typical of the cell cortex, and bundles as they extend along the axis of filopods, were recognized. A significant alteration found was an enhanced accumulation of actin in cytoskeletons of the mutant when cells were stimulated with cyclic AMP. Our results indicate that control of cell shape and motility does not require the fine-tuned interactions of all proteins that have been identified as actin-binding proteins by in vitro assays.

Original languageEnglish (US)
Pages (from-to)1477-1489
Number of pages13
JournalJournal of Cell Biology
Volume111
Issue number4
StatePublished - Oct 1990

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Dictyostelium
Actins
Microfilament Proteins
Proteins
Actin Cytoskeleton
Amoeba
Cell Shape
Cell Extracts
Spores
Cytoskeleton
Cyclic AMP
Cell Movement
Epitopes
Peptides
Antibodies
Growth

ASJC Scopus subject areas

  • Cell Biology

Cite this

Brink, M., Gerisch, G., Isenberg, G., Noegel, A. A., Segall, J. E., Wallraff, E., & Schleicher, M. (1990). A Dictyostelium mutant lacking an F-actin cross-linking protein, the 120-kD gelation factor. Journal of Cell Biology, 111(4), 1477-1489.

A Dictyostelium mutant lacking an F-actin cross-linking protein, the 120-kD gelation factor. / Brink, Marijke; Gerisch, Günther; Isenberg, Gerhard; Noegel, Angelika A.; Segall, Jeffrey E.; Wallraff, Eva; Schleicher, Michael.

In: Journal of Cell Biology, Vol. 111, No. 4, 10.1990, p. 1477-1489.

Research output: Contribution to journalArticle

Brink, M, Gerisch, G, Isenberg, G, Noegel, AA, Segall, JE, Wallraff, E & Schleicher, M 1990, 'A Dictyostelium mutant lacking an F-actin cross-linking protein, the 120-kD gelation factor', Journal of Cell Biology, vol. 111, no. 4, pp. 1477-1489.
Brink M, Gerisch G, Isenberg G, Noegel AA, Segall JE, Wallraff E et al. A Dictyostelium mutant lacking an F-actin cross-linking protein, the 120-kD gelation factor. Journal of Cell Biology. 1990 Oct;111(4):1477-1489.
Brink, Marijke ; Gerisch, Günther ; Isenberg, Gerhard ; Noegel, Angelika A. ; Segall, Jeffrey E. ; Wallraff, Eva ; Schleicher, Michael. / A Dictyostelium mutant lacking an F-actin cross-linking protein, the 120-kD gelation factor. In: Journal of Cell Biology. 1990 ; Vol. 111, No. 4. pp. 1477-1489.
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