@article{c085c4350da9436b8bae03ba9f8646e2,
title = "A developmental defect in astrocytes inhibits programmed regression of the hyaloid vasculature in the mammalian eye",
abstract = "Previously we reported the novel observation that astrocytes ensheath the persistent hyaloid artery, both in the Nuc1 spontaneous mutant rat, and in human PFV (persistent fetal vasculature) disease (Developmental Dynamics 234:36-47, 2005). We now show that astrocytes isolated from both the optic nerve and retina of Nuc1 rats migrate faster than wild type astrocytes. Aquaporin 4 (AQP4), the major water channel in astrocytes, has been shown to be important in astrocyte migration. We demonstrate that AQP4 expression is elevated in the astrocytes in PFV conditions, and we hypothesize that this causes the cells to migrate abnormally into the vitreous where they ensheath the hyaloid artery. This abnormal association of astrocytes with the hyaloid artery may impede the normal macrophage-mediated remodeling and regression of the hyaloid system.",
keywords = "Aquaporin-4 (AQP4), Astrocytes, Hyaloid vascular system, Migration, Optic nerve and retina, βA3/A1-crystallin",
author = "Cheng Zhang and Laura Asnaghi and Celine Gongora and Bonnie Patek and Stacey Hose and Bo Ma and Fard, {Masoud Aghsaei} and Lawrence Brako and Kamaljeet Singh and Goldberg, {Morton F.} and Handa, {James T.} and Lo, {Woo Kuen} and Eberhart, {Charles G.} and Zigler, {J. Samuel} and Debasish Sinha",
note = "Funding Information: This work was supported by grants from the National Institutes of Health : EY018636 (DS), EY019037 (DS), EY019037-S (DS), EY05314 (WKL), EY01765 (Wilmer Imaging Core), Intramural Research Program , National Eye Institute (JSZ) , Helena Rubinstein Foundation (DS) , Research to Prevent Blindness (an unrestricted grant to The Wilmer Eye Institute). We are grateful to Dr. Peter Nemeth, University of Pecs, Hungary, for providing the Aquaporin-4 monoclonal antibody used in this study. We thank Tanya Malpic-Ilanos for brain astrocyte cultures, Rhonda Grebe for confocal microscopy, Vaishnavi Srihar and Katayoon B. Ebrahimi for proliferation and migration assays, and to the Staff Members at Spring Valley Laboratories, Woodbine, MD, for taking care of the experimental animals. We thank Drs. Bhaja Padhi, Gerard A. Lutty and Edward Ratovitski for critical reading and discussion regarding this manuscript. ",
year = "2011",
month = may,
doi = "10.1016/j.ejcb.2011.01.003",
language = "English (US)",
volume = "90",
pages = "440--448",
journal = "European Journal of Cell Biology",
issn = "0171-9335",
publisher = "Urban und Fischer Verlag GmbH und Co. KG",
number = "5",
}
