A cytotoxic proteinase isolated from human lymphocytes

Victor Bernard Hatcher, M. S. Oberman, G. S. Lazarus, A. I. Grayzel

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

A proteinase active at physiologic pH was isolated from unstimulated human peripheral blood lymphocytes with gel filtration and affinity chromatography. The proteinase with a molecular mass of approximately 30,000 daltons was completely inhibited by diisopropylfluorophosphate (DFP) and soybean trypsin inhibitor (STI). Incubation of the lymphocyte enzyme with 3H-proline labeled T24 human bladder carcinoma cells resulted in significant cytotoxicity of the target cells. Cytotoxicity was only observed with much higher concentrations of trypsin. Similar results were obtained with a 51Cr release assay. This investigation demonstrates that unstimulated human peripheral blood lymphocytes contain a cytotoxic proteinase capable of killing pre-labeled target cells. The proteinase may be involved in lymphocyte-mediated cytotoxicity.

Original languageEnglish (US)
Pages (from-to)665-670
Number of pages6
JournalJournal of Immunology
Volume120
Issue number2
StatePublished - 1978

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Peptide Hydrolases
Lymphocytes
Isoflurophate
Trypsin Inhibitors
Affinity Chromatography
Soybeans
Proline
Trypsin
Gel Chromatography
Urinary Bladder
Carcinoma
Enzymes

ASJC Scopus subject areas

  • Immunology

Cite this

Hatcher, V. B., Oberman, M. S., Lazarus, G. S., & Grayzel, A. I. (1978). A cytotoxic proteinase isolated from human lymphocytes. Journal of Immunology, 120(2), 665-670.

A cytotoxic proteinase isolated from human lymphocytes. / Hatcher, Victor Bernard; Oberman, M. S.; Lazarus, G. S.; Grayzel, A. I.

In: Journal of Immunology, Vol. 120, No. 2, 1978, p. 665-670.

Research output: Contribution to journalArticle

Hatcher, VB, Oberman, MS, Lazarus, GS & Grayzel, AI 1978, 'A cytotoxic proteinase isolated from human lymphocytes', Journal of Immunology, vol. 120, no. 2, pp. 665-670.
Hatcher VB, Oberman MS, Lazarus GS, Grayzel AI. A cytotoxic proteinase isolated from human lymphocytes. Journal of Immunology. 1978;120(2):665-670.
Hatcher, Victor Bernard ; Oberman, M. S. ; Lazarus, G. S. ; Grayzel, A. I. / A cytotoxic proteinase isolated from human lymphocytes. In: Journal of Immunology. 1978 ; Vol. 120, No. 2. pp. 665-670.
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