A cross-sectional study of changes in markers of immunological effects and lung health due to exposure to multi-walled carbon nanotubes

Jelle Vlaanderen, Anjoeka Pronk, Nathaniel Rothman, Allan Hildesheim, Debra Silverman, Howard D. Hosgood, Suzanne Spaan, Eelco Kuijpers, Lode Godderis, Peter Hoet, Qing Lan, Roel Vermeulen

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Background: Multi-wall carbon nanotubes (MWCNTs) are manufactured nanomaterials to which workers and the general population will be increasingly exposed in coming years. Little is known about potential human health effects of exposure to MWCNTs, but effects on the lung and the immune system have been reported in animal and mechanistic studies. Objectives: We conducted a cross-sectional study to assess the association between occupational exposure to MWCNTs and effects on lung health and the immune system. Methods: We assessed 51 immune markers and three pneumoproteins in serum, complete blood cell counts (CBC), fractional exhaled nitric oxide (FENO), and lung function among 22 workers of a MWCNT producing facility and 39 age- and gender-matched, unexposed controls. Measurements were repeated four months later among 16 workers also included in the first phase of the study. Regression analyses were adjusted for potentially confounding parameters age, body mass index, smoking, and sex, and we explored potential confounding by other factors in sensitivity analyses. Results: We observed significant upward trends for immune markers C-C motif ligand 20 (p =.005), basic fibroblast growth factor (p =.05), and soluble IL-1 receptor II (p =.0004) with increasing exposure to MWCNT. These effects were replicated in the second phase of the study and were robust to sensitivity analyses. We also observed differences in FENO and several CBC parameters between exposed and non-exposed, but no difference in lung function or the pneumoproteins. Conclusions: We observed indications of early effects of occupational exposure to MWCNTs on lung health and the immune system.

Original languageEnglish (US)
Pages (from-to)395-404
Number of pages10
JournalNanotoxicology
Volume11
Issue number3
DOIs
StatePublished - Mar 16 2017

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Carbon Nanotubes
Carbon nanotubes
Blood Cell Count
Cross-Sectional Studies
Health
Lung
Immune system
Immune System
Nitric oxide
Occupational Exposure
Nitric Oxide
Blood
Biomarkers
Interleukin-1 Receptors
Nanostructures
Fibroblast Growth Factor 2
Fibroblasts
Nanostructured materials
Animals
Body Mass Index

Keywords

  • Carbon nanotubes
  • immune markers
  • lung health
  • nano particles
  • occupational exposure

ASJC Scopus subject areas

  • Biomedical Engineering
  • Toxicology

Cite this

A cross-sectional study of changes in markers of immunological effects and lung health due to exposure to multi-walled carbon nanotubes. / Vlaanderen, Jelle; Pronk, Anjoeka; Rothman, Nathaniel; Hildesheim, Allan; Silverman, Debra; Hosgood, Howard D.; Spaan, Suzanne; Kuijpers, Eelco; Godderis, Lode; Hoet, Peter; Lan, Qing; Vermeulen, Roel.

In: Nanotoxicology, Vol. 11, No. 3, 16.03.2017, p. 395-404.

Research output: Contribution to journalArticle

Vlaanderen, J, Pronk, A, Rothman, N, Hildesheim, A, Silverman, D, Hosgood, HD, Spaan, S, Kuijpers, E, Godderis, L, Hoet, P, Lan, Q & Vermeulen, R 2017, 'A cross-sectional study of changes in markers of immunological effects and lung health due to exposure to multi-walled carbon nanotubes', Nanotoxicology, vol. 11, no. 3, pp. 395-404. https://doi.org/10.1080/17435390.2017.1308031
Vlaanderen, Jelle ; Pronk, Anjoeka ; Rothman, Nathaniel ; Hildesheim, Allan ; Silverman, Debra ; Hosgood, Howard D. ; Spaan, Suzanne ; Kuijpers, Eelco ; Godderis, Lode ; Hoet, Peter ; Lan, Qing ; Vermeulen, Roel. / A cross-sectional study of changes in markers of immunological effects and lung health due to exposure to multi-walled carbon nanotubes. In: Nanotoxicology. 2017 ; Vol. 11, No. 3. pp. 395-404.
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AU - Hosgood, Howard D.

AU - Spaan, Suzanne

AU - Kuijpers, Eelco

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AU - Hoet, Peter

AU - Lan, Qing

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N2 - Background: Multi-wall carbon nanotubes (MWCNTs) are manufactured nanomaterials to which workers and the general population will be increasingly exposed in coming years. Little is known about potential human health effects of exposure to MWCNTs, but effects on the lung and the immune system have been reported in animal and mechanistic studies. Objectives: We conducted a cross-sectional study to assess the association between occupational exposure to MWCNTs and effects on lung health and the immune system. Methods: We assessed 51 immune markers and three pneumoproteins in serum, complete blood cell counts (CBC), fractional exhaled nitric oxide (FENO), and lung function among 22 workers of a MWCNT producing facility and 39 age- and gender-matched, unexposed controls. Measurements were repeated four months later among 16 workers also included in the first phase of the study. Regression analyses were adjusted for potentially confounding parameters age, body mass index, smoking, and sex, and we explored potential confounding by other factors in sensitivity analyses. Results: We observed significant upward trends for immune markers C-C motif ligand 20 (p =.005), basic fibroblast growth factor (p =.05), and soluble IL-1 receptor II (p =.0004) with increasing exposure to MWCNT. These effects were replicated in the second phase of the study and were robust to sensitivity analyses. We also observed differences in FENO and several CBC parameters between exposed and non-exposed, but no difference in lung function or the pneumoproteins. Conclusions: We observed indications of early effects of occupational exposure to MWCNTs on lung health and the immune system.

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