A competitive serological assay shows naturally acquired immunity to human papillomavirus infections in the guanacaste natural history study

Nicolas Wentzensen, Ana Cecilia Rodriguez, Raphael Viscidi, Rolando Herrero, Allan Hildesheim, Arpita Ghosh, Jorge Morales, Sholom Wacholder, Diego Guillen, Mario Alfaro, Mahboobeh Safaeian, Robert D. Burk, Mark Schiffman

Research output: Contribution to journalArticle

44 Scopus citations

Abstract

Background. A competitive Luminex Immunoassay (cLIA) has been developed to measure neutralizing antibodies against human papillomavirus (HPV) types 6, 11, 16 and 18. Methods. In a cohort of 974 women from the Guanacaste Natural History Study, we studied the relationship of baseline cLIA and virus-like particle (VLP) enzyme-linked immunosorbent assay (ELISA) (HPV16 and HPV18 only) seropositivity to measures of HPV exposure, HPV DNA positivity, number of sexual partners, cytology findings, and age. We then studied immunity against subsequent infection with HPV6, 11, 16, 18 and related types over a 7-year period. Results. cLIA seroprevalence varied with previous exposure; the prevalence of cLIA results positive for HPV16 and HPV18 was lower than the prevalence of positive VLP ELISA responses. cLIA and VLP ELISA positivity predicted protection from subsequent infections with concordant types. The combined odds ratio for HPV16 and HPV18 cLIA positivity was 0.41 (95% confidence interval [CI], 0.21-0.80), and the combined odds ratio for the HPV16 and HPV18 VLP ELISA positivity was 0.65 (95% CI, 0.46-0.93). Of individual types, statistical significance was only reached for HPV16 cLIA positivity (odds ratio, 0.44; 95% CI, 0.15-0.94). Conclusions. Both assays showed an association between positive results and significant protection from subsequent infections for HPV16 and HPV18 combined. cLIA seroprevalence was lower than VLP ELISA, suggesting that the assay detects a subset of antibodies following natural infection that are specifically linked to immunity against subsequent HPV infection.

Original languageEnglish (US)
Pages (from-to)94-102
Number of pages9
JournalJournal of Infectious Diseases
Volume204
Issue number1
DOIs
StatePublished - Jul 1 2011

ASJC Scopus subject areas

  • Immunology and Allergy
  • Infectious Diseases

Fingerprint Dive into the research topics of 'A competitive serological assay shows naturally acquired immunity to human papillomavirus infections in the guanacaste natural history study'. Together they form a unique fingerprint.

  • Cite this

    Wentzensen, N., Rodriguez, A. C., Viscidi, R., Herrero, R., Hildesheim, A., Ghosh, A., Morales, J., Wacholder, S., Guillen, D., Alfaro, M., Safaeian, M., Burk, R. D., & Schiffman, M. (2011). A competitive serological assay shows naturally acquired immunity to human papillomavirus infections in the guanacaste natural history study. Journal of Infectious Diseases, 204(1), 94-102. https://doi.org/10.1093/infdis/jir209