A comparison of the biocompatibility of phosphate-buffered saline and dianeal 3.86% in the rat model of peritoneal dialysis.

K. Wieczorowska-Tobis, A. Polubinska, A. Breborowicz, D. G. Oreopoulos

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Abstract

Phosphate-buffered saline (PBS), an isotonic solution with a physiologic pH can be considered an example of a biocompatible dialysis fluid. This study compared the biocompatibility of PBS with that of Dianeal 3.86% (Baxter Healthcare Corporation, Deerfield, IL, U.S.A.), using a model of peritoneal dialysis in the rat. In an acute experiment, after catheter implantation, rats were infused on day 1 with PBS, on day 5 with standard dialysis solution (Dianeal 3.86%), and on day 7 again with PBS. When rats were injected with Dianeal 3.86%, the inflammatory reaction was suppressed as compared with PBS. The cell count was lower with Dianeal (-85%, p < 0.001), the neutrophil:macrophage ratio in dialysate was 80% lower (p < 0.01), total protein concentration in the Dianeal dialysate was 73% lower (p < 0.01), and the dialysate nitrite level was 45% lower (p < 0.01). In a chronic experiment, after catheter implantation, rats were dialyzed for four weeks with PBS or with Dianeal 3.86%. At the end of the study, a 1-hour peritoneal equilibration test (PET) was performed. As evaluated on a semiquantitative scale, macroscopic changes in the peritoneum were more severe in rats exposed to PBS than in those exposed to Dianeal 3.86% (8.6 +/- 3.2 vs 5.2 +/- 2.6, p < 0.05). The thickness of the visceral peritoneum was comparable in both groups; but, in PBS-treated rats, the peritoneal interstitium contained more inflammatory cells and more new vessels. During the 1-hour PET, peritoneal permeability to water and solutes was comparable in the two groups. Despite a more physiologic composition, PBS is a less biocompatible peritoneal dialysis solutions than is standard, acidic, hypertonic dialysis solution.

Original languageEnglish (US)
Pages (from-to)42-46
Number of pages5
JournalAdvances in peritoneal dialysis. Conference on Peritoneal Dialysis
Volume17
StatePublished - 2001
Externally publishedYes

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Peritoneal Dialysis
Dialysis Solutions
Phosphates
Peritoneum
Catheters
Hypertonic Solutions
Isotonic Solutions
Nitrites
Sodium Chloride
Dialysis
Permeability
Neutrophils
Cell Count
Macrophages
Delivery of Health Care
Water

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A comparison of the biocompatibility of phosphate-buffered saline and dianeal 3.86% in the rat model of peritoneal dialysis. / Wieczorowska-Tobis, K.; Polubinska, A.; Breborowicz, A.; Oreopoulos, D. G.

In: Advances in peritoneal dialysis. Conference on Peritoneal Dialysis, Vol. 17, 2001, p. 42-46.

Research output: Contribution to journalArticle

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