A comparison of snRNP-associated Sm-autoantigens: Human N, rat N and human B/B′

Claudia Schmauss, George Mcallister, Yasuo Ohosone, John A. Hardin, Michael R. Lerner

Research output: Contribution to journalArticle

50 Citations (Scopus)

Abstract

N is a tissue-specific, Sm-epitope bearing, snRNP-associated protein found predominantly in brain. The cDNA sequence encoding human N is compared to those for rat N and human B/B′. The amino acid sequences of human and rat N are 100% conserved. Although the amino acid sequences of N and B/B′ are very similar to each other, B7sol;B′ contains 50 amino acids which are not present in N. On Northern blots the cDNAs encoding N and B/B′ recognize two different RNA species. A comparison of the codon usage, as specified by the open reading frames of N and B/B′ as well as results from Southern blots, show that N and B/B′ are derived from different genes.

Original languageEnglish (US)
Pages (from-to)1733-1743
Number of pages11
JournalNucleic Acids Research
Volume17
Issue number4
DOIs
StatePublished - Feb 25 1989
Externally publishedYes

Fingerprint

Small Nuclear Ribonucleoproteins
Autoantigens
Amino acids
Rats
Amino Acid Sequence
CDNA
Amino Acids
Encoding
Bearings (structural)
Complementary DNA
Epitopes
Southern Blotting
RNA
Codon
Northern Blotting
Open Reading Frames
Brain
Genes
Tissue
Gene

ASJC Scopus subject areas

  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Health, Toxicology and Mutagenesis
  • Toxicology
  • Genetics(clinical)
  • Genetics

Cite this

Schmauss, C., Mcallister, G., Ohosone, Y., Hardin, J. A., & Lerner, M. R. (1989). A comparison of snRNP-associated Sm-autoantigens: Human N, rat N and human B/B′. Nucleic Acids Research, 17(4), 1733-1743. https://doi.org/10.1093/nar/17.4.1733

A comparison of snRNP-associated Sm-autoantigens : Human N, rat N and human B/B′. / Schmauss, Claudia; Mcallister, George; Ohosone, Yasuo; Hardin, John A.; Lerner, Michael R.

In: Nucleic Acids Research, Vol. 17, No. 4, 25.02.1989, p. 1733-1743.

Research output: Contribution to journalArticle

Schmauss, C, Mcallister, G, Ohosone, Y, Hardin, JA & Lerner, MR 1989, 'A comparison of snRNP-associated Sm-autoantigens: Human N, rat N and human B/B′', Nucleic Acids Research, vol. 17, no. 4, pp. 1733-1743. https://doi.org/10.1093/nar/17.4.1733
Schmauss, Claudia ; Mcallister, George ; Ohosone, Yasuo ; Hardin, John A. ; Lerner, Michael R. / A comparison of snRNP-associated Sm-autoantigens : Human N, rat N and human B/B′. In: Nucleic Acids Research. 1989 ; Vol. 17, No. 4. pp. 1733-1743.
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