A comparison of snRNP-associated Sm-autoantigens: Human N, rat N and human B/B′

Claudia Schmauss, George Mcallister, Yasuo Ohosone, John A. Hardin, Michael R. Lerner

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Abstract

N is a tissue-specific, Sm-epitope bearing, snRNP-associated protein found predominantly in brain. The cDNA sequence encoding human N is compared to those for rat N and human B/B′. The amino acid sequences of human and rat N are 100% conserved. Although the amino acid sequences of N and B/B′ are very similar to each other, B7sol;B′ contains 50 amino acids which are not present in N. On Northern blots the cDNAs encoding N and B/B′ recognize two different RNA species. A comparison of the codon usage, as specified by the open reading frames of N and B/B′ as well as results from Southern blots, show that N and B/B′ are derived from different genes.

Original languageEnglish (US)
Pages (from-to)1733-1743
Number of pages11
JournalNucleic acids research
Volume17
Issue number4
DOIs
StatePublished - Feb 25 1989

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ASJC Scopus subject areas

  • Genetics

Cite this

Schmauss, C., Mcallister, G., Ohosone, Y., Hardin, J. A., & Lerner, M. R. (1989). A comparison of snRNP-associated Sm-autoantigens: Human N, rat N and human B/B′. Nucleic acids research, 17(4), 1733-1743. https://doi.org/10.1093/nar/17.4.1733