A comparison of linear array and hybrid capture 2 for detection of carcinogenic human papillomavirus and cervical precancer in ASCUS-LSIL Triage Study

Patti E. Gravitt, Mark Schiffman, Diane Solomon, Cosette M. Wheeler, Philip E. Castle

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

Background: We were interested in comparing the performance of Linear Array (LA; Roche Molecular Systems) to Hybrid Capture 2 (hc2; Digene) for the detection of carcinogenic human papillomavirus (HPV) and cervical precancer. Methods: LA and hc2 results were compared on baseline specimens collected from women with an atypical squamous cells of undetermined significance (ASCUS) Pap referred into ASCUS and Low-Grade Squamous Intraepithelial Lesion Triage Study (n = 3,488). hc2 was conducted at the time of the study on liquid cytology specimens. LA was conducted retrospectively on aliquots from a second, stored cervical specimen masked to the hc2 results and clinical data. Paired LA and hc2 results (n = 3,289; 94%) were compared for the detection of carcinogenic HPV (HPV 16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, 59, 66, and 68) and 2-year cumulative cervical intraepithelial neoplasia (CIN) grade ≥3 as diagnosed by the quality-control pathology review. Results: LA was more likely to test positive for carcinogenic HPV than hc2 (55% versus 53%; P = 0.001). For 2-year cumulative ≥CIN3, LA and hc2 had similar sensitivities (93.3% versus 92.6%, respectively; P = 1), and LA was marginally less specific than hc2 (48.1% versus 50.6%, respectively; P = 0.05). LA and hc2 had similar negative predictive values (98.70% versus 98.64% respectively; P = 0.4), and LA had a slightly lower positive predictive value than hc2 (14.6% versus 15.1%, respectively; P < 0.0001). Conclusion: We observed that LA and hc2 performed similarly in the detection of carcinogenic HPV and identification of CIN3 among women with an ASCUS Pap.

Original languageEnglish (US)
Pages (from-to)1248-1254
Number of pages7
JournalCancer Epidemiology Biomarkers and Prevention
Volume17
Issue number5
DOIs
StatePublished - May 2008
Externally publishedYes

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Triage
Human papillomavirus 18
Forensic Anthropology
Cervical Intraepithelial Neoplasia
Time and Motion Studies
Human papillomavirus 16
Quality Control
Cell Biology
Pathology
Atypical Squamous Cells of the Cervix

ASJC Scopus subject areas

  • Epidemiology
  • Oncology

Cite this

A comparison of linear array and hybrid capture 2 for detection of carcinogenic human papillomavirus and cervical precancer in ASCUS-LSIL Triage Study. / Gravitt, Patti E.; Schiffman, Mark; Solomon, Diane; Wheeler, Cosette M.; Castle, Philip E.

In: Cancer Epidemiology Biomarkers and Prevention, Vol. 17, No. 5, 05.2008, p. 1248-1254.

Research output: Contribution to journalArticle

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title = "A comparison of linear array and hybrid capture 2 for detection of carcinogenic human papillomavirus and cervical precancer in ASCUS-LSIL Triage Study",
abstract = "Background: We were interested in comparing the performance of Linear Array (LA; Roche Molecular Systems) to Hybrid Capture 2 (hc2; Digene) for the detection of carcinogenic human papillomavirus (HPV) and cervical precancer. Methods: LA and hc2 results were compared on baseline specimens collected from women with an atypical squamous cells of undetermined significance (ASCUS) Pap referred into ASCUS and Low-Grade Squamous Intraepithelial Lesion Triage Study (n = 3,488). hc2 was conducted at the time of the study on liquid cytology specimens. LA was conducted retrospectively on aliquots from a second, stored cervical specimen masked to the hc2 results and clinical data. Paired LA and hc2 results (n = 3,289; 94{\%}) were compared for the detection of carcinogenic HPV (HPV 16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, 59, 66, and 68) and 2-year cumulative cervical intraepithelial neoplasia (CIN) grade ≥3 as diagnosed by the quality-control pathology review. Results: LA was more likely to test positive for carcinogenic HPV than hc2 (55{\%} versus 53{\%}; P = 0.001). For 2-year cumulative ≥CIN3, LA and hc2 had similar sensitivities (93.3{\%} versus 92.6{\%}, respectively; P = 1), and LA was marginally less specific than hc2 (48.1{\%} versus 50.6{\%}, respectively; P = 0.05). LA and hc2 had similar negative predictive values (98.70{\%} versus 98.64{\%} respectively; P = 0.4), and LA had a slightly lower positive predictive value than hc2 (14.6{\%} versus 15.1{\%}, respectively; P < 0.0001). Conclusion: We observed that LA and hc2 performed similarly in the detection of carcinogenic HPV and identification of CIN3 among women with an ASCUS Pap.",
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T1 - A comparison of linear array and hybrid capture 2 for detection of carcinogenic human papillomavirus and cervical precancer in ASCUS-LSIL Triage Study

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