A common RNA recognition motif identified within a defined U1 RNA binding domain of the 70K U1 snRNP protein

Charles C. Query, Rex C. Bentley, Jack D. Keene

Research output: Contribution to journalArticle

432 Citations (Scopus)

Abstract

We have defined the RNA binding domain of the 70K protein component of the U1 small nuclear ribonucleoprotein to a region of 111 amino acids. This domain encompasses an octamer sequence that has been observed in other proteins associated with RNA, but has not previously been shown to bind directly to a specific RNA sequence. Within the U1 RNA binding domain, an 80 amino acid consensus sequence that is conserved in many presumed RNA binding proteins was discerned. This sequence pattern appears to represent an RNA recognition motif (RRM) characteristic of a distinct family of proteins. By site-directed mutagenesis, we determined that the 70K protein consists of 437 amino acids (52 kd), and found that its aberrant electrophoretic migration is due to a carboxy-terminal charged domain structurally similar to two Drosophila proteins (su(wa) and tra) that may regulate alternative pre-messenger RNA splicing.

Original languageEnglish (US)
Pages (from-to)89-101
Number of pages13
JournalCell
Volume57
Issue number1
DOIs
StatePublished - Apr 7 1989
Externally publishedYes

Fingerprint

U1 Small Nuclear Ribonucleoproteins
RNA
Amino Acids
RNA Splicing
Drosophila Proteins
Proteins
RNA-Binding Proteins
Consensus Sequence
Site-Directed Mutagenesis
Mutagenesis
Amino Acid Sequence
Messenger RNA
RNA Recognition Motif
U1 small nuclear RNA
RNA-Binding Motifs

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology

Cite this

A common RNA recognition motif identified within a defined U1 RNA binding domain of the 70K U1 snRNP protein. / Query, Charles C.; Bentley, Rex C.; Keene, Jack D.

In: Cell, Vol. 57, No. 1, 07.04.1989, p. 89-101.

Research output: Contribution to journalArticle

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