A commercial preparation of catalase inhibits nitric oxide production by activated murine macrophages

Role of arginase

Y. Tian, Y. Xing, R. Magliozzo, K. Yu, B. R. Bloom, John Chan

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Catalase is widely used as a pharmacological probe to evaluate the role of hydrogen peroxide in antimicrobial activities of phagocytic cells. This report demonstrates that the ability of a commercial preparation of catalase to inhibit concomitantly macrophage antimycobacterial activity and production of reactive nitrogen intermediates can be attributed, at least in part, to the depletion of L-arginine by contaminating arginase. In experimental systems that employ pharmacological probes, the existence of nonspecific effects should be considered in data interpretation.

Original languageEnglish (US)
Pages (from-to)3015-3018
Number of pages4
JournalInfection and Immunity
Volume68
Issue number5
DOIs
StatePublished - May 2000

Fingerprint

Arginase
Catalase
Nitric Oxide
Macrophages
Pharmacology
Phagocytes
Hydrogen Peroxide
Arginine
Nitrogen

ASJC Scopus subject areas

  • Immunology

Cite this

A commercial preparation of catalase inhibits nitric oxide production by activated murine macrophages : Role of arginase. / Tian, Y.; Xing, Y.; Magliozzo, R.; Yu, K.; Bloom, B. R.; Chan, John.

In: Infection and Immunity, Vol. 68, No. 5, 05.2000, p. 3015-3018.

Research output: Contribution to journalArticle

Tian, Y. ; Xing, Y. ; Magliozzo, R. ; Yu, K. ; Bloom, B. R. ; Chan, John. / A commercial preparation of catalase inhibits nitric oxide production by activated murine macrophages : Role of arginase. In: Infection and Immunity. 2000 ; Vol. 68, No. 5. pp. 3015-3018.
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