A commercial preparation of catalase inhibits nitric oxide production by activated murine macrophages: Role of arginase

Y. Tian, Y. Xing, R. Magliozzo, K. Yu, B. R. Bloom, J. Chan

Research output: Contribution to journalArticle

8 Scopus citations

Abstract

Catalase is widely used as a pharmacological probe to evaluate the role of hydrogen peroxide in antimicrobial activities of phagocytic cells. This report demonstrates that the ability of a commercial preparation of catalase to inhibit concomitantly macrophage antimycobacterial activity and production of reactive nitrogen intermediates can be attributed, at least in part, to the depletion of L-arginine by contaminating arginase. In experimental systems that employ pharmacological probes, the existence of nonspecific effects should be considered in data interpretation.

Original languageEnglish (US)
Pages (from-to)3015-3018
Number of pages4
JournalInfection and Immunity
Volume68
Issue number5
DOIs
Publication statusPublished - May 1 2000

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ASJC Scopus subject areas

  • Parasitology
  • Microbiology
  • Immunology
  • Infectious Diseases

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