A combination of exercise and capsinoid supplementation additively suppresses diet-induced obesity by increasing energy expenditure in mice

Kana Ohyama, Yoshihito Nogusa, Katsuya Suzuki, Kosaku Shinoda, Shingo Kajimura, Makoto Bannai

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Exercise effectively prevents the development of obesity and obesity-related diseases such as type 2 diabetes. Capsinoids (CSNs) are capsaicin analogs found in a nonpungent pepper that increase whole body energy expenditure. Although both exercise and CSNs have antiobesity functions, the effectiveness of exercise with CSN supplementation has not yet been investigated. Here, we examined whether the beneficial effects of exercise could be further enhanced by CSN supplementation in mice. Mice were randomly assigned to four groups: 1) high-fat diet (HFD, Control), 2) HFD containing 0.3% CSNs, 3) HFD with voluntary running wheel exercise (Exercise), and 4) HFD containing 0.3% CSNs with voluntary running wheel exercise (Exercise + CSN). After 8 wk of ingestion, blood and tissues were collected and analyzed. Although CSNs significantly suppressed body weight gain under the HFD, CSN supplementation with exercise additively decreased body weight gain and fat accumulation and increased whole body energy expenditure compared with exercise alone. Exercise together with CSN supplementation robustly improved metabolic profiles, including the plasma cholesterol level. Furthermore, this combination significantly prevented diet-induced liver steatosis and decreased the size of adipocyte cells in white adipose tissue. Exercise and CSNs significantly increased cAMP levels and PKA activity in brown adipose tissue (BAT), indicating an increase of lipolysis. Moreover, they significantly activated both the oxidative phosphorylation gene program and fatty acid oxidation in skeletal muscle. These results indicate that CSNs efficiently promote the antiobesity effect of exercise, in part by increasing energy expenditure via the activation of fat oxidation in skeletal muscle and lipolysis in BAT.

Original languageEnglish (US)
Pages (from-to)E315-E323
JournalAmerican Journal of Physiology - Endocrinology and Metabolism
Volume308
Issue number4
DOIs
StatePublished - Jan 1 2015
Externally publishedYes

Fingerprint

Energy Metabolism
Brown Adipose Tissue
Obesity
Lipolysis
Diet
Running
Weight Gain
Skeletal Muscle
Body Weight
White Adipose Tissue
Metabolome
Capsaicin
Oxidative Phosphorylation
High Fat Diet
Fatty Liver
Cell Size
Adipocytes
Type 2 Diabetes Mellitus
Adipose Tissue
Fatty Acids

Keywords

  • Capsinoids
  • Energy expenditure
  • Exercise
  • Fat oxidation
  • Obesity

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Physiology
  • Physiology (medical)

Cite this

A combination of exercise and capsinoid supplementation additively suppresses diet-induced obesity by increasing energy expenditure in mice. / Ohyama, Kana; Nogusa, Yoshihito; Suzuki, Katsuya; Shinoda, Kosaku; Kajimura, Shingo; Bannai, Makoto.

In: American Journal of Physiology - Endocrinology and Metabolism, Vol. 308, No. 4, 01.01.2015, p. E315-E323.

Research output: Contribution to journalArticle

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