TY - JOUR
T1 - A cluster of VanD vancomycin-resistant Enterococcus faecium
T2 - Molecular characterization and clinical epidemiology
AU - Ostrowsky, Belinda E.
AU - Clark, Nancye C.
AU - Thauvin-Eliopoulos, Claudie
AU - Venkataraman, Lata
AU - Samore, Matthew H.
AU - Tenover, Fred C.
AU - Eliopoulos, George M.
AU - Moellering, Robert C.
AU - Gold, Howard S.
N1 - Funding Information:
Financial support: Centers for Disease Control (T01/CCT11438, Postdoctoral Fellowship Training Program in Infectious Diseases, to B.E.O.); Infectious Diseases Society of America (young investigator award to H.S.G.); William F. Milton Fund.
PY - 1999
Y1 - 1999
N2 - VanD-mediated glycopeptide resistance has been reported for an isolate of Enterococcus faecium, BM4339. Three clinical isolates of vancomycin- resistant E. faecium collected from 3 patients during a 6-week period in 1993 had agar dilution MICs of vancomycin and teicoplanin of 128 and 4 μg/mL, respectively. Polymerase chain reaction (PCR) using degenerate primers complementary to genes encoding D-Ala-D-X ligases yielded a 630-bp product that was similar to the published partial sequence of vanD. By use of inverse PCR, vanD, vanH(D), and two partial flanking open-reading frames were sequenced. The deduced amino acid sequence of VanD showed 67% identity with VanA and VanB. vanD appeared to be located on the chromosome and was not transferable to other enterococci. The 3 isolates were indistinguishable by pulsed-field gel electrophoresis and differed from BM4339. No other isolates carrying rand were found in a subset of 875 recent US isolates of vancomycin- resistant enterococci.
AB - VanD-mediated glycopeptide resistance has been reported for an isolate of Enterococcus faecium, BM4339. Three clinical isolates of vancomycin- resistant E. faecium collected from 3 patients during a 6-week period in 1993 had agar dilution MICs of vancomycin and teicoplanin of 128 and 4 μg/mL, respectively. Polymerase chain reaction (PCR) using degenerate primers complementary to genes encoding D-Ala-D-X ligases yielded a 630-bp product that was similar to the published partial sequence of vanD. By use of inverse PCR, vanD, vanH(D), and two partial flanking open-reading frames were sequenced. The deduced amino acid sequence of VanD showed 67% identity with VanA and VanB. vanD appeared to be located on the chromosome and was not transferable to other enterococci. The 3 isolates were indistinguishable by pulsed-field gel electrophoresis and differed from BM4339. No other isolates carrying rand were found in a subset of 875 recent US isolates of vancomycin- resistant enterococci.
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U2 - 10.1086/315030
DO - 10.1086/315030
M3 - Article
C2 - 10479146
AN - SCOPUS:0033394802
SN - 0022-1899
VL - 180
SP - 1177
EP - 1185
JO - Journal of Infectious Diseases
JF - Journal of Infectious Diseases
IS - 4
ER -
