A cluster of VanD vancomycin-resistant Enterococcus faecium: Molecular characterization and clinical epidemiology

Belinda E. Ostrowsky, Nancye C. Clark, Claudie Thauvin-Eliopoulos, Lata Venkataraman, Matthew H. Samore, Fred C. Tenover, George M. Eliopoulos, Robert C. Moellering, Howard S. Gold

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

VanD-mediated glycopeptide resistance has been reported for an isolate of Enterococcus faecium, BM4339. Three clinical isolates of vancomycin- resistant E. faecium collected from 3 patients during a 6-week period in 1993 had agar dilution MICs of vancomycin and teicoplanin of 128 and 4 μg/mL, respectively. Polymerase chain reaction (PCR) using degenerate primers complementary to genes encoding D-Ala-D-X ligases yielded a 630-bp product that was similar to the published partial sequence of vanD. By use of inverse PCR, vanD, vanH(D), and two partial flanking open-reading frames were sequenced. The deduced amino acid sequence of VanD showed 67% identity with VanA and VanB. vanD appeared to be located on the chromosome and was not transferable to other enterococci. The 3 isolates were indistinguishable by pulsed-field gel electrophoresis and differed from BM4339. No other isolates carrying rand were found in a subset of 875 recent US isolates of vancomycin- resistant enterococci.

Original languageEnglish (US)
Pages (from-to)1177-1185
Number of pages9
JournalJournal of Infectious Diseases
Volume180
Issue number4
DOIs
StatePublished - 1999
Externally publishedYes

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Enterococcus faecium
Epidemiology
Teicoplanin
Polymerase Chain Reaction
Glycopeptides
Pulsed Field Gel Electrophoresis
Enterococcus
Vancomycin
Ligases
Open Reading Frames
Agar
Amino Acid Sequence
Chromosomes
Genes
Vancomycin-Resistant Enterococci

ASJC Scopus subject areas

  • Public Health, Environmental and Occupational Health
  • Immunology

Cite this

Ostrowsky, B. E., Clark, N. C., Thauvin-Eliopoulos, C., Venkataraman, L., Samore, M. H., Tenover, F. C., ... Gold, H. S. (1999). A cluster of VanD vancomycin-resistant Enterococcus faecium: Molecular characterization and clinical epidemiology. Journal of Infectious Diseases, 180(4), 1177-1185. https://doi.org/10.1086/315030

A cluster of VanD vancomycin-resistant Enterococcus faecium : Molecular characterization and clinical epidemiology. / Ostrowsky, Belinda E.; Clark, Nancye C.; Thauvin-Eliopoulos, Claudie; Venkataraman, Lata; Samore, Matthew H.; Tenover, Fred C.; Eliopoulos, George M.; Moellering, Robert C.; Gold, Howard S.

In: Journal of Infectious Diseases, Vol. 180, No. 4, 1999, p. 1177-1185.

Research output: Contribution to journalArticle

Ostrowsky, BE, Clark, NC, Thauvin-Eliopoulos, C, Venkataraman, L, Samore, MH, Tenover, FC, Eliopoulos, GM, Moellering, RC & Gold, HS 1999, 'A cluster of VanD vancomycin-resistant Enterococcus faecium: Molecular characterization and clinical epidemiology', Journal of Infectious Diseases, vol. 180, no. 4, pp. 1177-1185. https://doi.org/10.1086/315030
Ostrowsky BE, Clark NC, Thauvin-Eliopoulos C, Venkataraman L, Samore MH, Tenover FC et al. A cluster of VanD vancomycin-resistant Enterococcus faecium: Molecular characterization and clinical epidemiology. Journal of Infectious Diseases. 1999;180(4):1177-1185. https://doi.org/10.1086/315030
Ostrowsky, Belinda E. ; Clark, Nancye C. ; Thauvin-Eliopoulos, Claudie ; Venkataraman, Lata ; Samore, Matthew H. ; Tenover, Fred C. ; Eliopoulos, George M. ; Moellering, Robert C. ; Gold, Howard S. / A cluster of VanD vancomycin-resistant Enterococcus faecium : Molecular characterization and clinical epidemiology. In: Journal of Infectious Diseases. 1999 ; Vol. 180, No. 4. pp. 1177-1185.
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