A cluster of VanD vancomycin-resistant Enterococcus faecium: Molecular characterization and clinical epidemiology

Belinda E. Ostrowsky, Nancye C. Clark, Claudie Thauvin-Eliopoulos, Lata Venkataraman, Matthew H. Samore, Fred C. Tenover, George M. Eliopoulos, Robert C. Moellering, Howard S. Gold

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37 Scopus citations

Abstract

VanD-mediated glycopeptide resistance has been reported for an isolate of Enterococcus faecium, BM4339. Three clinical isolates of vancomycin- resistant E. faecium collected from 3 patients during a 6-week period in 1993 had agar dilution MICs of vancomycin and teicoplanin of 128 and 4 μg/mL, respectively. Polymerase chain reaction (PCR) using degenerate primers complementary to genes encoding D-Ala-D-X ligases yielded a 630-bp product that was similar to the published partial sequence of vanD. By use of inverse PCR, vanD, vanH(D), and two partial flanking open-reading frames were sequenced. The deduced amino acid sequence of VanD showed 67% identity with VanA and VanB. vanD appeared to be located on the chromosome and was not transferable to other enterococci. The 3 isolates were indistinguishable by pulsed-field gel electrophoresis and differed from BM4339. No other isolates carrying rand were found in a subset of 875 recent US isolates of vancomycin- resistant enterococci.

Original languageEnglish (US)
Pages (from-to)1177-1185
Number of pages9
JournalJournal of Infectious Diseases
Volume180
Issue number4
DOIs
StatePublished - Dec 1 1999

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ASJC Scopus subject areas

  • Immunology and Allergy
  • Infectious Diseases

Cite this

Ostrowsky, B. E., Clark, N. C., Thauvin-Eliopoulos, C., Venkataraman, L., Samore, M. H., Tenover, F. C., Eliopoulos, G. M., Moellering, R. C., & Gold, H. S. (1999). A cluster of VanD vancomycin-resistant Enterococcus faecium: Molecular characterization and clinical epidemiology. Journal of Infectious Diseases, 180(4), 1177-1185. https://doi.org/10.1086/315030