A case of sporotrichosis caused by different Sporothrix brasiliensis strains: mycological, molecular, and virulence analyses

Manoel Marques E. Oliveira, Rodrigo Almeida-Paes, Danielly Corrêa-Moreira, Cintia de Moraes Borba, Rodrigo Caldas Menezes, Dayvison Francis Saraiva Freitas, Antonio Carlos Francesconi do Valle, Armando de Oliveira Schubach, Monica Bastos de Lima Barros, Joshua D. Nosanchuk, Maria Clara Gutierrez-Galhardo, Rosely Maria Zancopé-Oliveira

Research output: Contribution to journalArticle

Abstract

BACKGROUND: Sporotrichosis is a subcutaneous mycosis caused by dimorphic pathogenic fungi belonging to the Sporothrix genus. Pathogenic Sporothrix species typically produce melanin, which is known to be a virulence factor. OBJECTIVES: The aim of this study was to perform phenotypic, genotypic, and virulence analyses of two distinct Sporothrix brasiliensis strains isolated from the same lesion on a patient from Rio de Janeiro. METHODS AND FINDINGS: Genotypic analyses by partial sequencing of the calmodulin, β-tubulin, and chitin synthase genes, as well as polymerase chain reaction (PCR)-fingerprinting by T3B, M13, and GACA, showed that the isolates were very similar but not identical. Both isolates had similar phenotypic characteristics and effectively produced melanin in their yeast forms, accounting for their ability of causing disease in a murine sporotrichosis model. Remarkably, isolate B was albino in its environmental form but caused more severe disease than the pigmented A isolate. CONCLUSIONS: These findings indicate that the patient was infected by two genetically and biologically distinct S. brasiliensis that vary in their production of melanin in their environmental forms. The results underscore the importance of characterizing phenotypically different isolates found in the same clinical specimen or patient.

Original languageEnglish (US)
Pages (from-to)e190260
JournalMemorias do Instituto Oswaldo Cruz
Volume114
DOIs
StatePublished - Jan 1 2019

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Sporothrix
Sporotrichosis
Melanins
Virulence
Chitin Synthase
Mycoses
Virulence Factors
Calmodulin
Tubulin
Fungi
Yeasts
Polymerase Chain Reaction
Genes

ASJC Scopus subject areas

  • Microbiology (medical)

Cite this

Oliveira, M. M. E., Almeida-Paes, R., Corrêa-Moreira, D., Borba, C. D. M., Menezes, R. C., Freitas, D. F. S., ... Zancopé-Oliveira, R. M. (2019). A case of sporotrichosis caused by different Sporothrix brasiliensis strains: mycological, molecular, and virulence analyses. Memorias do Instituto Oswaldo Cruz, 114, e190260. https://doi.org/10.1590/0074-02760190260

A case of sporotrichosis caused by different Sporothrix brasiliensis strains : mycological, molecular, and virulence analyses. / Oliveira, Manoel Marques E.; Almeida-Paes, Rodrigo; Corrêa-Moreira, Danielly; Borba, Cintia de Moraes; Menezes, Rodrigo Caldas; Freitas, Dayvison Francis Saraiva; do Valle, Antonio Carlos Francesconi; Schubach, Armando de Oliveira; Barros, Monica Bastos de Lima; Nosanchuk, Joshua D.; Gutierrez-Galhardo, Maria Clara; Zancopé-Oliveira, Rosely Maria.

In: Memorias do Instituto Oswaldo Cruz, Vol. 114, 01.01.2019, p. e190260.

Research output: Contribution to journalArticle

Oliveira, MME, Almeida-Paes, R, Corrêa-Moreira, D, Borba, CDM, Menezes, RC, Freitas, DFS, do Valle, ACF, Schubach, ADO, Barros, MBDL, Nosanchuk, JD, Gutierrez-Galhardo, MC & Zancopé-Oliveira, RM 2019, 'A case of sporotrichosis caused by different Sporothrix brasiliensis strains: mycological, molecular, and virulence analyses', Memorias do Instituto Oswaldo Cruz, vol. 114, pp. e190260. https://doi.org/10.1590/0074-02760190260
Oliveira, Manoel Marques E. ; Almeida-Paes, Rodrigo ; Corrêa-Moreira, Danielly ; Borba, Cintia de Moraes ; Menezes, Rodrigo Caldas ; Freitas, Dayvison Francis Saraiva ; do Valle, Antonio Carlos Francesconi ; Schubach, Armando de Oliveira ; Barros, Monica Bastos de Lima ; Nosanchuk, Joshua D. ; Gutierrez-Galhardo, Maria Clara ; Zancopé-Oliveira, Rosely Maria. / A case of sporotrichosis caused by different Sporothrix brasiliensis strains : mycological, molecular, and virulence analyses. In: Memorias do Instituto Oswaldo Cruz. 2019 ; Vol. 114. pp. e190260.
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