A bioMEMS device for the study of mechanical properties of cells

Joseph M. Sanders, Logan Butt, Ashley Clark, James Williams, Michael Padgen, Edison Leung, Patricia Keely, John S. Condeelis, Julio Aguirre-Ghiso, James Castracane

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

The tumor microenvironment is a complex system which is not fully understood. New technologies are needed to provide a better understanding of the role of the tumor microenvironment in promoting metastasis. The Nano Intravital Device, or NANIVID, has been developed as an optically transparent, implantable tool to study the tumor microenvironment. Two etched glass substrates are sealed using a thin polymer membrane to create a reservoir with a single outlet. This reservoir is loaded with a custom hydrogel blend that contains selected factors for delivery to the tumor microenvironment. When the device is implanted in the tumor, the hydrogel swells and releases these entrapped molecules, forming a sustained concentration gradient. The NANIVID has previously been successful in manipulating the tumor microenvironment both in vitro as well as in vivo. As metastatic cells intravasate, it has been shown that some are able to do so unscathed and reach their new location, while others are cleaved during the process<sup>1</sup>. There appears to be a correlation between cell migration and the mechanical properties of these cells. It is believed that these properties can be detected in real time by atomic force microscopy. In this study, metastatic MTLn3 rat mammary cells are seeded onto 1-dimensional microfibers and directed up a stable gradient of growth factor. The NANIVID device is placed behind our AFM tip, where it generates a stable chemotactic gradient of epidermal growth factor. Scanning confocal laser microscopy is also used to monitor movement of the cells over time. This experiment will shed light on the mechanical changes in metastatic cells as they undergo directed migration.

Original languageEnglish (US)
Title of host publicationProgress in Biomedical Optics and Imaging - Proceedings of SPIE
PublisherSPIE
Volume9320
ISBN (Print)9781628414103
DOIs
StatePublished - 2015
EventMicrofluidics, BioMEMS, and Medical Microsystems XIII - San Francisco, United States
Duration: Feb 7 2015Feb 9 2015

Other

OtherMicrofluidics, BioMEMS, and Medical Microsystems XIII
CountryUnited States
CitySan Francisco
Period2/7/152/9/15

Fingerprint

Tumor Microenvironment
Tumors
tumors
mechanical properties
Equipment and Supplies
Mechanical properties
cells
Hydrogel
Cell Movement
Hydrogels
gradients
atomic force microscopy
Atomic Force Microscopy
laser microscopy
microfibers
Epidermal Growth Factor
Confocal Microscopy
metastasis
Glass
outlets

Keywords

  • atomic force microscopy
  • breast cancer
  • chemotaxis
  • mechanical properties
  • metastasis
  • NANIVID

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics
  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Radiology Nuclear Medicine and imaging

Cite this

Sanders, J. M., Butt, L., Clark, A., Williams, J., Padgen, M., Leung, E., ... Castracane, J. (2015). A bioMEMS device for the study of mechanical properties of cells. In Progress in Biomedical Optics and Imaging - Proceedings of SPIE (Vol. 9320). [932017] SPIE. https://doi.org/10.1117/12.2078521

A bioMEMS device for the study of mechanical properties of cells. / Sanders, Joseph M.; Butt, Logan; Clark, Ashley; Williams, James; Padgen, Michael; Leung, Edison; Keely, Patricia; Condeelis, John S.; Aguirre-Ghiso, Julio; Castracane, James.

Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 9320 SPIE, 2015. 932017.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Sanders, JM, Butt, L, Clark, A, Williams, J, Padgen, M, Leung, E, Keely, P, Condeelis, JS, Aguirre-Ghiso, J & Castracane, J 2015, A bioMEMS device for the study of mechanical properties of cells. in Progress in Biomedical Optics and Imaging - Proceedings of SPIE. vol. 9320, 932017, SPIE, Microfluidics, BioMEMS, and Medical Microsystems XIII, San Francisco, United States, 2/7/15. https://doi.org/10.1117/12.2078521
Sanders JM, Butt L, Clark A, Williams J, Padgen M, Leung E et al. A bioMEMS device for the study of mechanical properties of cells. In Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 9320. SPIE. 2015. 932017 https://doi.org/10.1117/12.2078521
Sanders, Joseph M. ; Butt, Logan ; Clark, Ashley ; Williams, James ; Padgen, Michael ; Leung, Edison ; Keely, Patricia ; Condeelis, John S. ; Aguirre-Ghiso, Julio ; Castracane, James. / A bioMEMS device for the study of mechanical properties of cells. Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 9320 SPIE, 2015.
@inproceedings{663541d6862045338379e6089b3bc1c5,
title = "A bioMEMS device for the study of mechanical properties of cells",
abstract = "The tumor microenvironment is a complex system which is not fully understood. New technologies are needed to provide a better understanding of the role of the tumor microenvironment in promoting metastasis. The Nano Intravital Device, or NANIVID, has been developed as an optically transparent, implantable tool to study the tumor microenvironment. Two etched glass substrates are sealed using a thin polymer membrane to create a reservoir with a single outlet. This reservoir is loaded with a custom hydrogel blend that contains selected factors for delivery to the tumor microenvironment. When the device is implanted in the tumor, the hydrogel swells and releases these entrapped molecules, forming a sustained concentration gradient. The NANIVID has previously been successful in manipulating the tumor microenvironment both in vitro as well as in vivo. As metastatic cells intravasate, it has been shown that some are able to do so unscathed and reach their new location, while others are cleaved during the process1. There appears to be a correlation between cell migration and the mechanical properties of these cells. It is believed that these properties can be detected in real time by atomic force microscopy. In this study, metastatic MTLn3 rat mammary cells are seeded onto 1-dimensional microfibers and directed up a stable gradient of growth factor. The NANIVID device is placed behind our AFM tip, where it generates a stable chemotactic gradient of epidermal growth factor. Scanning confocal laser microscopy is also used to monitor movement of the cells over time. This experiment will shed light on the mechanical changes in metastatic cells as they undergo directed migration.",
keywords = "atomic force microscopy, breast cancer, chemotaxis, mechanical properties, metastasis, NANIVID",
author = "Sanders, {Joseph M.} and Logan Butt and Ashley Clark and James Williams and Michael Padgen and Edison Leung and Patricia Keely and Condeelis, {John S.} and Julio Aguirre-Ghiso and James Castracane",
year = "2015",
doi = "10.1117/12.2078521",
language = "English (US)",
isbn = "9781628414103",
volume = "9320",
booktitle = "Progress in Biomedical Optics and Imaging - Proceedings of SPIE",
publisher = "SPIE",

}

TY - GEN

T1 - A bioMEMS device for the study of mechanical properties of cells

AU - Sanders, Joseph M.

AU - Butt, Logan

AU - Clark, Ashley

AU - Williams, James

AU - Padgen, Michael

AU - Leung, Edison

AU - Keely, Patricia

AU - Condeelis, John S.

AU - Aguirre-Ghiso, Julio

AU - Castracane, James

PY - 2015

Y1 - 2015

N2 - The tumor microenvironment is a complex system which is not fully understood. New technologies are needed to provide a better understanding of the role of the tumor microenvironment in promoting metastasis. The Nano Intravital Device, or NANIVID, has been developed as an optically transparent, implantable tool to study the tumor microenvironment. Two etched glass substrates are sealed using a thin polymer membrane to create a reservoir with a single outlet. This reservoir is loaded with a custom hydrogel blend that contains selected factors for delivery to the tumor microenvironment. When the device is implanted in the tumor, the hydrogel swells and releases these entrapped molecules, forming a sustained concentration gradient. The NANIVID has previously been successful in manipulating the tumor microenvironment both in vitro as well as in vivo. As metastatic cells intravasate, it has been shown that some are able to do so unscathed and reach their new location, while others are cleaved during the process1. There appears to be a correlation between cell migration and the mechanical properties of these cells. It is believed that these properties can be detected in real time by atomic force microscopy. In this study, metastatic MTLn3 rat mammary cells are seeded onto 1-dimensional microfibers and directed up a stable gradient of growth factor. The NANIVID device is placed behind our AFM tip, where it generates a stable chemotactic gradient of epidermal growth factor. Scanning confocal laser microscopy is also used to monitor movement of the cells over time. This experiment will shed light on the mechanical changes in metastatic cells as they undergo directed migration.

AB - The tumor microenvironment is a complex system which is not fully understood. New technologies are needed to provide a better understanding of the role of the tumor microenvironment in promoting metastasis. The Nano Intravital Device, or NANIVID, has been developed as an optically transparent, implantable tool to study the tumor microenvironment. Two etched glass substrates are sealed using a thin polymer membrane to create a reservoir with a single outlet. This reservoir is loaded with a custom hydrogel blend that contains selected factors for delivery to the tumor microenvironment. When the device is implanted in the tumor, the hydrogel swells and releases these entrapped molecules, forming a sustained concentration gradient. The NANIVID has previously been successful in manipulating the tumor microenvironment both in vitro as well as in vivo. As metastatic cells intravasate, it has been shown that some are able to do so unscathed and reach their new location, while others are cleaved during the process1. There appears to be a correlation between cell migration and the mechanical properties of these cells. It is believed that these properties can be detected in real time by atomic force microscopy. In this study, metastatic MTLn3 rat mammary cells are seeded onto 1-dimensional microfibers and directed up a stable gradient of growth factor. The NANIVID device is placed behind our AFM tip, where it generates a stable chemotactic gradient of epidermal growth factor. Scanning confocal laser microscopy is also used to monitor movement of the cells over time. This experiment will shed light on the mechanical changes in metastatic cells as they undergo directed migration.

KW - atomic force microscopy

KW - breast cancer

KW - chemotaxis

KW - mechanical properties

KW - metastasis

KW - NANIVID

UR - http://www.scopus.com/inward/record.url?scp=84931841021&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84931841021&partnerID=8YFLogxK

U2 - 10.1117/12.2078521

DO - 10.1117/12.2078521

M3 - Conference contribution

AN - SCOPUS:84931841021

SN - 9781628414103

VL - 9320

BT - Progress in Biomedical Optics and Imaging - Proceedings of SPIE

PB - SPIE

ER -