A 45-kDa protein antigenically related to band 3 is selectively expressed in kidney mitochondria

Lynda S. Ostedgaard, Michael L. Jennings, Lawrence P. Karniski, Victor L. Schuster

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Anion exchange similar to that catalyzed by erythrocyte band 3 occurs across many nonerythroid cell membranes. To identify anton-exchange proteins structurally related to band 3, we immunobtotted rabbit kidney medullary membrane fractions with anti-band 3 antibodies. Immunoblots using antibodies to the cytoplasmic domain of band 3 revealed cross-reactive proteins in the plasma membrane fraction only. In contrast, two monoclonal antibodies against band 3 membrane domain labeled a 45-kDa protein; further immunoblotting and immunogold studies of membrane fractions and kidney sections using one of the anti-membrane domain antibodies showed that labeling was strongest in mitochondria of H+-secreting collecting duct cells. Tissue-to-tissue expression of the 45-kDa mitochondrial protein was variable: kidney medulla > heart > kidney cortex ≫ liver. We conclude that a 45-kDa protein with immunological cross-reactivity to the erythrocyte band 3 membrane domain is expressed in mitochondria in a highly cell-specific fashion and speculate that the protein may play a role in mitochondrial anion transport. (.

Original languageEnglish (US)
Pages (from-to)981-985
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume88
Issue number3
StatePublished - 1991

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Mitochondria
Kidney
Membranes
Proteins
Anions
Antibodies
Erythrocytes
Cell Membrane
Kidney Medulla
Kidney Cortex
Mitochondrial Proteins
Immunoblotting
Monoclonal Antibodies
Rabbits
Liver

Keywords

  • Anion exchange
  • Renal acidification

ASJC Scopus subject areas

  • General
  • Genetics

Cite this

A 45-kDa protein antigenically related to band 3 is selectively expressed in kidney mitochondria. / Ostedgaard, Lynda S.; Jennings, Michael L.; Karniski, Lawrence P.; Schuster, Victor L.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 88, No. 3, 1991, p. 981-985.

Research output: Contribution to journalArticle

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AB - Anion exchange similar to that catalyzed by erythrocyte band 3 occurs across many nonerythroid cell membranes. To identify anton-exchange proteins structurally related to band 3, we immunobtotted rabbit kidney medullary membrane fractions with anti-band 3 antibodies. Immunoblots using antibodies to the cytoplasmic domain of band 3 revealed cross-reactive proteins in the plasma membrane fraction only. In contrast, two monoclonal antibodies against band 3 membrane domain labeled a 45-kDa protein; further immunoblotting and immunogold studies of membrane fractions and kidney sections using one of the anti-membrane domain antibodies showed that labeling was strongest in mitochondria of H+-secreting collecting duct cells. Tissue-to-tissue expression of the 45-kDa mitochondrial protein was variable: kidney medulla > heart > kidney cortex ≫ liver. We conclude that a 45-kDa protein with immunological cross-reactivity to the erythrocyte band 3 membrane domain is expressed in mitochondria in a highly cell-specific fashion and speculate that the protein may play a role in mitochondrial anion transport. (.

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