TY - JOUR
T1 - A 2.8-Mb clone contig of the multiple endocrine neoplasia type 1 (MEN1) region at 11q13
AU - Guru, Siradanahalli C.
AU - Olufemi, Shodimu Emmanuel
AU - Manickam, Pachiappan
AU - Cummings, Christiano
AU - Gieser, Linn M.
AU - Pike, Brian L.
AU - Bittner, Michael L.
AU - Jiang, Yuan
AU - Chinault, A. Craig
AU - Nowak, Norma J.
AU - Brzozowska, Anna
AU - Crabtree, Judy S.
AU - Wang, Yingping
AU - Roe, Bruce
AU - Weisemann, Jane M.
AU - Boguski, Mark S.
AU - Agarwal, Sunita K.
AU - Burns, A. Lee
AU - Spiegele, Allen M.
AU - Marx, Stephen J.
AU - Rejter, Wendy L.
AU - De Jong, Pieter J.
AU - Collins, Francis S.
AU - Chandrasekharappa, Settara C.
N1 - Funding Information:
We thank Rakesh Anand for the ICI YAC library, Larry Deaven for the chromosome 11 cosmid library, Carol Jones for somatic cell hybrids, Eri Srivatsan for VNTR cosmids, and Christina Robbins and Darryl Leja for help with sequencing and illustrations. We also acknowledge a DOE graduate fellowship to J.S.C. and support from the intramural research programs of NHGRI, NIDDK, and NLM.
PY - 1997/6/15
Y1 - 1997/6/15
N2 - Multiple endocrine neoplasia type 1 (MEN1) is an autosomal dominant disorder that results in parathyroid, anterior pituitary, and pancreatic and duodenal endocrine tumors in affected individuals. The MEN1 locus is tightly linked to the marker PYGM on chromosome 11q13, and linkage analysis has placed the MEN1 gene within a 2-Mb interval flanked by D11S1883 and D11S449. As a step toward cloning the MEN1 gene, we have constructed a 2.8-Mb clone contig consisting of YAC and bacterial clones (PAC, BAC, and P1) for the D11S480 to D11S913 region. The bacterial clones alone represent nearly all of the 2.8-Mb contig. The contig was assembled based on a high-density STS- content analysis of 79 genomic clones (YAC, PAC, BAC, and P1) with 118 STSs. The STSs included 22 polymorphic markers and 20 transcripts, with the remainder primarily derived from the end sequences of the genomic clones. An independent cosmid contig for the 1-Mb PYGM-SEA region was also generated. Support for correctness of the 2.8-Mb contig map comes from an independent ordering of the clones by fiber-FiSH. This sequence-ready contig will be a useful resource for positional cloning of MEN1 and other disease genes whose loci fall within this region.
AB - Multiple endocrine neoplasia type 1 (MEN1) is an autosomal dominant disorder that results in parathyroid, anterior pituitary, and pancreatic and duodenal endocrine tumors in affected individuals. The MEN1 locus is tightly linked to the marker PYGM on chromosome 11q13, and linkage analysis has placed the MEN1 gene within a 2-Mb interval flanked by D11S1883 and D11S449. As a step toward cloning the MEN1 gene, we have constructed a 2.8-Mb clone contig consisting of YAC and bacterial clones (PAC, BAC, and P1) for the D11S480 to D11S913 region. The bacterial clones alone represent nearly all of the 2.8-Mb contig. The contig was assembled based on a high-density STS- content analysis of 79 genomic clones (YAC, PAC, BAC, and P1) with 118 STSs. The STSs included 22 polymorphic markers and 20 transcripts, with the remainder primarily derived from the end sequences of the genomic clones. An independent cosmid contig for the 1-Mb PYGM-SEA region was also generated. Support for correctness of the 2.8-Mb contig map comes from an independent ordering of the clones by fiber-FiSH. This sequence-ready contig will be a useful resource for positional cloning of MEN1 and other disease genes whose loci fall within this region.
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U2 - 10.1006/geno.1997.4783
DO - 10.1006/geno.1997.4783
M3 - Article
C2 - 9205115
AN - SCOPUS:0031570711
SN - 0888-7543
VL - 42
SP - 436
EP - 445
JO - Genomics
JF - Genomics
IS - 3
ER -