A 2.8-Mb clone contig of the multiple endocrine neoplasia type 1 (MEN1) region at 11q13

Siradanahalli C. Guru, Shodimu Emmanuel Olufemi, Pachiappan Manickam, Christiano Cummings, Linn M. Gieser, Brian L. Pike, Michael L. Bittner, Yuan Jiang, A. Craig Chinault, Norma J. Nowak, Anna Brzozowska, Judy S. Crabtree, Yingping Wang, Bruce Roe, Jane M. Weisemann, Mark S. Boguski, Sunita K. Agarwal, A. Lee Burns, Allen M. Spiegele, Stephen J. MarxWendy L. Rejter, Pieter J. De Jong, Francis S. Collins, Settara C. Chandrasekharappa

Research output: Contribution to journalArticle

37 Scopus citations

Abstract

Multiple endocrine neoplasia type 1 (MEN1) is an autosomal dominant disorder that results in parathyroid, anterior pituitary, and pancreatic and duodenal endocrine tumors in affected individuals. The MEN1 locus is tightly linked to the marker PYGM on chromosome 11q13, and linkage analysis has placed the MEN1 gene within a 2-Mb interval flanked by D11S1883 and D11S449. As a step toward cloning the MEN1 gene, we have constructed a 2.8-Mb clone contig consisting of YAC and bacterial clones (PAC, BAC, and P1) for the D11S480 to D11S913 region. The bacterial clones alone represent nearly all of the 2.8-Mb contig. The contig was assembled based on a high-density STS- content analysis of 79 genomic clones (YAC, PAC, BAC, and P1) with 118 STSs. The STSs included 22 polymorphic markers and 20 transcripts, with the remainder primarily derived from the end sequences of the genomic clones. An independent cosmid contig for the 1-Mb PYGM-SEA region was also generated. Support for correctness of the 2.8-Mb contig map comes from an independent ordering of the clones by fiber-FiSH. This sequence-ready contig will be a useful resource for positional cloning of MEN1 and other disease genes whose loci fall within this region.

Original languageEnglish (US)
Pages (from-to)436-445
Number of pages10
JournalGenomics
Volume42
Issue number3
DOIs
StatePublished - Jun 15 1997
Externally publishedYes

    Fingerprint

ASJC Scopus subject areas

  • Genetics

Cite this

Guru, S. C., Olufemi, S. E., Manickam, P., Cummings, C., Gieser, L. M., Pike, B. L., Bittner, M. L., Jiang, Y., Chinault, A. C., Nowak, N. J., Brzozowska, A., Crabtree, J. S., Wang, Y., Roe, B., Weisemann, J. M., Boguski, M. S., Agarwal, S. K., Burns, A. L., Spiegele, A. M., ... Chandrasekharappa, S. C. (1997). A 2.8-Mb clone contig of the multiple endocrine neoplasia type 1 (MEN1) region at 11q13. Genomics, 42(3), 436-445. https://doi.org/10.1006/geno.1997.4783