Abstract
5,5′-Dimethyloxazolidine-2,4-dione (DMO), a weak non-metabolizable acid, is commonly utilized for determining intracellular pH. In these studies, DMO was tested as an inducer of differentiation on the basis that its uptake and subsequent dissociation might transiently raise intracellular pH and activate ion-fluxes critical for triggering maturation. After 5 days of exposure to 40 mM DMO, >60% of HL-60 cells displayed phenotypic and functional changes characteristic of mature granulocytes. As with other inducers of HL-60 cell differentiation, commitment to differentiation required culture in the presence of DMO for more than 24 h, indicating that if transient effects on pH or ion-fluxes occurred, they were not sufficient to trigger this process. DMO was either weak or inactive as an inducer of murine erythroleukemia cell (FLC) differentiation. Although other weak acids and bases triggered differentiation of both HL-60 cells and FLC, the spectrum of response differed markedly between the two lines. These results suggest that: (1) a number of common buffering agents have the potential to alter cell phenotype, and (2) their effects must be evaluated for each individual cell type.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 181-190 |
| Number of pages | 10 |
| Journal | Cell Differentiation and Development |
| Volume | 26 |
| Issue number | 3 |
| DOIs | |
| State | Published - 1989 |
| Externally published | Yes |
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Keywords
- 5,5′-Dimethyloxazolidine-2,4-dione
- Cellular differentiation
- Friend erythroleukemia cell
- Human promyelocytic leukemia cell
- Ion-flux
- Neutrophil
- Poly ADP ribosylation
ASJC Scopus subject areas
- Developmental Biology
Cite this
5,5′-dimethyloxazolidine-2,4-dione is a strong inducer of differentiation of human promyelocytic leukemia (HL-60) cells. / Calderon, Tina M.; Schneiderman, Natalie; Michl, Josef; Christman, Judith K.
In: Cell Differentiation and Development, Vol. 26, No. 3, 1989, p. 181-190.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - 5,5′-dimethyloxazolidine-2,4-dione is a strong inducer of differentiation of human promyelocytic leukemia (HL-60) cells
AU - Calderon, Tina M.
AU - Schneiderman, Natalie
AU - Michl, Josef
AU - Christman, Judith K.
PY - 1989
Y1 - 1989
N2 - 5,5′-Dimethyloxazolidine-2,4-dione (DMO), a weak non-metabolizable acid, is commonly utilized for determining intracellular pH. In these studies, DMO was tested as an inducer of differentiation on the basis that its uptake and subsequent dissociation might transiently raise intracellular pH and activate ion-fluxes critical for triggering maturation. After 5 days of exposure to 40 mM DMO, >60% of HL-60 cells displayed phenotypic and functional changes characteristic of mature granulocytes. As with other inducers of HL-60 cell differentiation, commitment to differentiation required culture in the presence of DMO for more than 24 h, indicating that if transient effects on pH or ion-fluxes occurred, they were not sufficient to trigger this process. DMO was either weak or inactive as an inducer of murine erythroleukemia cell (FLC) differentiation. Although other weak acids and bases triggered differentiation of both HL-60 cells and FLC, the spectrum of response differed markedly between the two lines. These results suggest that: (1) a number of common buffering agents have the potential to alter cell phenotype, and (2) their effects must be evaluated for each individual cell type.
AB - 5,5′-Dimethyloxazolidine-2,4-dione (DMO), a weak non-metabolizable acid, is commonly utilized for determining intracellular pH. In these studies, DMO was tested as an inducer of differentiation on the basis that its uptake and subsequent dissociation might transiently raise intracellular pH and activate ion-fluxes critical for triggering maturation. After 5 days of exposure to 40 mM DMO, >60% of HL-60 cells displayed phenotypic and functional changes characteristic of mature granulocytes. As with other inducers of HL-60 cell differentiation, commitment to differentiation required culture in the presence of DMO for more than 24 h, indicating that if transient effects on pH or ion-fluxes occurred, they were not sufficient to trigger this process. DMO was either weak or inactive as an inducer of murine erythroleukemia cell (FLC) differentiation. Although other weak acids and bases triggered differentiation of both HL-60 cells and FLC, the spectrum of response differed markedly between the two lines. These results suggest that: (1) a number of common buffering agents have the potential to alter cell phenotype, and (2) their effects must be evaluated for each individual cell type.
KW - 5,5′-Dimethyloxazolidine-2,4-dione
KW - Cellular differentiation
KW - Friend erythroleukemia cell
KW - Human promyelocytic leukemia cell
KW - Ion-flux
KW - Neutrophil
KW - Poly ADP ribosylation
UR - http://www.scopus.com/inward/record.url?scp=0024354882&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0024354882&partnerID=8YFLogxK
U2 - 10.1016/0922-3371(89)90749-1
DO - 10.1016/0922-3371(89)90749-1
M3 - Article
C2 - 2766039
AN - SCOPUS:0024354882
VL - 26
SP - 181
EP - 190
JO - Mechanisms of Development
JF - Mechanisms of Development
SN - 0925-4773
IS - 3
ER -