2,5-Hexanedione alters elemental composition and water content of rat peripheral nerve myelinated axons

Richard M. LoPachin, Ellen J. Lehning, Edward C. Stack, Steven J. Hussein, Albert J. Saubermann

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Effects of 2,5-hexanedione on elemental concentrations and water content of peripheral nerve myelinated axons were determined using electron probe x- ray microanalysis. Axons (small, medium, and large) were analyzed in unfixed cryosections from rat tibial and proximal sciatic nerve samples. Animals were intoxicated with 2,5-hexanedione by two dosing paradigms: intraperitoneal or oral. Regardless of the route of exposure, internodal axoplasm of small and medium axons from both nerve regions exhibited selective, progressive reductions in dry weight K concentrations and water content. When calculated on a wet weight basis, K levels were comparable to or slightly above control values in tibial nerve, whereas in sciatic nerve, small transient decreases in wet weight K were evident. These changes in K and water correlated with the development of axonal atrophy. The wet and dry weight internodal elemental changes reported here do not suggest a metabolic or axolemmal defect, but rather imply a homeostatic response possibly related to the process of axonal atrophy. Giant axonal swellings were primarily associated with oral 2,5-hexanedione intoxication, and corresponding analyses revealed few changes in element or water content compared with control. The absence of significant alterations in these swellings is consistent with mechanical expansion of the axon probably as a function of accumulating neurofilaments.

Original languageEnglish (US)
Pages (from-to)2266-2278
Number of pages13
JournalJournal of Neurochemistry
Volume63
Issue number6
StatePublished - Dec 1994
Externally publishedYes

Fingerprint

Peripheral Nerves
Water content
Axons
Rats
Weights and Measures
Water
Sciatic Nerve
Chemical analysis
Atrophy
Swelling
Tibial Nerve
Intermediate Filaments
Microanalysis
Animals
X-Rays
Electrons
X rays
Defects
2,5-hexanedione

Keywords

  • 2,5-Hexanedione
  • Chemical neurotoxicant
  • Distal axonopathy
  • Electron probe x-ray microanalysis
  • Myelinated axon

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

Cite this

LoPachin, R. M., Lehning, E. J., Stack, E. C., Hussein, S. J., & Saubermann, A. J. (1994). 2,5-Hexanedione alters elemental composition and water content of rat peripheral nerve myelinated axons. Journal of Neurochemistry, 63(6), 2266-2278.

2,5-Hexanedione alters elemental composition and water content of rat peripheral nerve myelinated axons. / LoPachin, Richard M.; Lehning, Ellen J.; Stack, Edward C.; Hussein, Steven J.; Saubermann, Albert J.

In: Journal of Neurochemistry, Vol. 63, No. 6, 12.1994, p. 2266-2278.

Research output: Contribution to journalArticle

LoPachin, RM, Lehning, EJ, Stack, EC, Hussein, SJ & Saubermann, AJ 1994, '2,5-Hexanedione alters elemental composition and water content of rat peripheral nerve myelinated axons', Journal of Neurochemistry, vol. 63, no. 6, pp. 2266-2278.
LoPachin, Richard M. ; Lehning, Ellen J. ; Stack, Edward C. ; Hussein, Steven J. ; Saubermann, Albert J. / 2,5-Hexanedione alters elemental composition and water content of rat peripheral nerve myelinated axons. In: Journal of Neurochemistry. 1994 ; Vol. 63, No. 6. pp. 2266-2278.
@article{054b4b107088449baf0c84976df31dd8,
title = "2,5-Hexanedione alters elemental composition and water content of rat peripheral nerve myelinated axons",
abstract = "Effects of 2,5-hexanedione on elemental concentrations and water content of peripheral nerve myelinated axons were determined using electron probe x- ray microanalysis. Axons (small, medium, and large) were analyzed in unfixed cryosections from rat tibial and proximal sciatic nerve samples. Animals were intoxicated with 2,5-hexanedione by two dosing paradigms: intraperitoneal or oral. Regardless of the route of exposure, internodal axoplasm of small and medium axons from both nerve regions exhibited selective, progressive reductions in dry weight K concentrations and water content. When calculated on a wet weight basis, K levels were comparable to or slightly above control values in tibial nerve, whereas in sciatic nerve, small transient decreases in wet weight K were evident. These changes in K and water correlated with the development of axonal atrophy. The wet and dry weight internodal elemental changes reported here do not suggest a metabolic or axolemmal defect, but rather imply a homeostatic response possibly related to the process of axonal atrophy. Giant axonal swellings were primarily associated with oral 2,5-hexanedione intoxication, and corresponding analyses revealed few changes in element or water content compared with control. The absence of significant alterations in these swellings is consistent with mechanical expansion of the axon probably as a function of accumulating neurofilaments.",
keywords = "2,5-Hexanedione, Chemical neurotoxicant, Distal axonopathy, Electron probe x-ray microanalysis, Myelinated axon",
author = "LoPachin, {Richard M.} and Lehning, {Ellen J.} and Stack, {Edward C.} and Hussein, {Steven J.} and Saubermann, {Albert J.}",
year = "1994",
month = "12",
language = "English (US)",
volume = "63",
pages = "2266--2278",
journal = "Journal of Neurochemistry",
issn = "0022-3042",
publisher = "Wiley-Blackwell",
number = "6",

}

TY - JOUR

T1 - 2,5-Hexanedione alters elemental composition and water content of rat peripheral nerve myelinated axons

AU - LoPachin, Richard M.

AU - Lehning, Ellen J.

AU - Stack, Edward C.

AU - Hussein, Steven J.

AU - Saubermann, Albert J.

PY - 1994/12

Y1 - 1994/12

N2 - Effects of 2,5-hexanedione on elemental concentrations and water content of peripheral nerve myelinated axons were determined using electron probe x- ray microanalysis. Axons (small, medium, and large) were analyzed in unfixed cryosections from rat tibial and proximal sciatic nerve samples. Animals were intoxicated with 2,5-hexanedione by two dosing paradigms: intraperitoneal or oral. Regardless of the route of exposure, internodal axoplasm of small and medium axons from both nerve regions exhibited selective, progressive reductions in dry weight K concentrations and water content. When calculated on a wet weight basis, K levels were comparable to or slightly above control values in tibial nerve, whereas in sciatic nerve, small transient decreases in wet weight K were evident. These changes in K and water correlated with the development of axonal atrophy. The wet and dry weight internodal elemental changes reported here do not suggest a metabolic or axolemmal defect, but rather imply a homeostatic response possibly related to the process of axonal atrophy. Giant axonal swellings were primarily associated with oral 2,5-hexanedione intoxication, and corresponding analyses revealed few changes in element or water content compared with control. The absence of significant alterations in these swellings is consistent with mechanical expansion of the axon probably as a function of accumulating neurofilaments.

AB - Effects of 2,5-hexanedione on elemental concentrations and water content of peripheral nerve myelinated axons were determined using electron probe x- ray microanalysis. Axons (small, medium, and large) were analyzed in unfixed cryosections from rat tibial and proximal sciatic nerve samples. Animals were intoxicated with 2,5-hexanedione by two dosing paradigms: intraperitoneal or oral. Regardless of the route of exposure, internodal axoplasm of small and medium axons from both nerve regions exhibited selective, progressive reductions in dry weight K concentrations and water content. When calculated on a wet weight basis, K levels were comparable to or slightly above control values in tibial nerve, whereas in sciatic nerve, small transient decreases in wet weight K were evident. These changes in K and water correlated with the development of axonal atrophy. The wet and dry weight internodal elemental changes reported here do not suggest a metabolic or axolemmal defect, but rather imply a homeostatic response possibly related to the process of axonal atrophy. Giant axonal swellings were primarily associated with oral 2,5-hexanedione intoxication, and corresponding analyses revealed few changes in element or water content compared with control. The absence of significant alterations in these swellings is consistent with mechanical expansion of the axon probably as a function of accumulating neurofilaments.

KW - 2,5-Hexanedione

KW - Chemical neurotoxicant

KW - Distal axonopathy

KW - Electron probe x-ray microanalysis

KW - Myelinated axon

UR - http://www.scopus.com/inward/record.url?scp=0027946631&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027946631&partnerID=8YFLogxK

M3 - Article

C2 - 7964747

AN - SCOPUS:0027946631

VL - 63

SP - 2266

EP - 2278

JO - Journal of Neurochemistry

JF - Journal of Neurochemistry

SN - 0022-3042

IS - 6

ER -