244-251 IgM + memory B cell expression predicts HIV-associated cryptococcosis status

Krishanthi Subramaniam, Brian Metzger, Lawrence H. Hanau, Alice Guh, Lisa Rucker, Sheila Badri, Liise-anne Pirofski

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Abstract

Background. The role of B cells in resistance to Cryptococcus neoformans disease (i.e., cryptococcosis) is unknown. Given evidence that IgM + memory B cells are required for immunity to other encapsulated pathogens, we hypothesized that these cells might contribute to resistance to cryptococcosis. Methods. We compared levels of IgM expression on memory B cells in 29 HIV-infected individuals who had a history of cryptococcosis (the HIV+CN+ group) with levels in 30 human immunodeficiency virus (HlV)-infected subjects who had no history of cryptococcosis (the HIV+CN- group) and 20 HIV-uninfected subjects who had no history of cryptococcosis (the HIV- group) (cohort 1). We also determined levels of IgM expression on memory B cells in banked samples obtained before cryptococcosis onset from 31 participants in the Multicenter AIDS Cohort Study, of whom 8 had HIV infection and subsequently developed cryptococcosis (the HIV+CN+ group), 8 had HIV infection and did not develop cryptococcosis (the HIV+CN- group), and 15 did not have HIV infection and did not develop cryptococcosis (the HIV- group) (cohort 2). Results. In cohort 1, the percentage of memory B cells that expressed IgM was lower among HIV+CN+ subjects, compared with HIV+CN- subjects (P<.01) and HIV- subjects (P<.05); expression of IgM on =S50% of memory B cells was a significant predictor of C. neoformans disease status (odds ratio, 5.5; P = .03). In cohort 2, the percentage of memory B cells that expressed IgM was lower in HIV+CN+ subjects than in HIV+CNsubjects (P = .02) and HIV- subjects (P<.01); an IgM + memory B cell percentage of =538.5% was a significant predictor of future development of cryptococcosis (odds ratio, 14; P = .02). Conclusions. These findings suggest that HIV-infected persons in whom the percentage of memory B cells that express IgM is decreased might be at greater risk for the development of cryptococcosis.

Original languageEnglish (US)
Pages (from-to)244-251
Number of pages8
JournalJournal of Infectious Diseases
Volume200
Issue number2
DOIs
StatePublished - Jul 15 2009

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Cryptococcosis
Immunoglobulin M
B-Lymphocytes
HIV
HIV Infections
Cryptococcus neoformans
Odds Ratio

ASJC Scopus subject areas

  • Infectious Diseases
  • Immunology and Allergy

Cite this

244-251 IgM + memory B cell expression predicts HIV-associated cryptococcosis status. / Subramaniam, Krishanthi; Metzger, Brian; Hanau, Lawrence H.; Guh, Alice; Rucker, Lisa; Badri, Sheila; Pirofski, Liise-anne.

In: Journal of Infectious Diseases, Vol. 200, No. 2, 15.07.2009, p. 244-251.

Research output: Contribution to journalArticle

Subramaniam, K, Metzger, B, Hanau, LH, Guh, A, Rucker, L, Badri, S & Pirofski, L 2009, '244-251 IgM + memory B cell expression predicts HIV-associated cryptococcosis status', Journal of Infectious Diseases, vol. 200, no. 2, pp. 244-251. https://doi.org/10.1086/599318
Subramaniam K, Metzger B, Hanau LH, Guh A, Rucker L, Badri S et al. 244-251 IgM + memory B cell expression predicts HIV-associated cryptococcosis status. Journal of Infectious Diseases. 2009 Jul 15;200(2):244-251. https://doi.org/10.1086/599318
Subramaniam, Krishanthi ; Metzger, Brian ; Hanau, Lawrence H. ; Guh, Alice ; Rucker, Lisa ; Badri, Sheila ; Pirofski, Liise-anne. / 244-251 IgM + memory B cell expression predicts HIV-associated cryptococcosis status. In: Journal of Infectious Diseases. 2009 ; Vol. 200, No. 2. pp. 244-251.
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abstract = "Background. The role of B cells in resistance to Cryptococcus neoformans disease (i.e., cryptococcosis) is unknown. Given evidence that IgM + memory B cells are required for immunity to other encapsulated pathogens, we hypothesized that these cells might contribute to resistance to cryptococcosis. Methods. We compared levels of IgM expression on memory B cells in 29 HIV-infected individuals who had a history of cryptococcosis (the HIV+CN+ group) with levels in 30 human immunodeficiency virus (HlV)-infected subjects who had no history of cryptococcosis (the HIV+CN- group) and 20 HIV-uninfected subjects who had no history of cryptococcosis (the HIV- group) (cohort 1). We also determined levels of IgM expression on memory B cells in banked samples obtained before cryptococcosis onset from 31 participants in the Multicenter AIDS Cohort Study, of whom 8 had HIV infection and subsequently developed cryptococcosis (the HIV+CN+ group), 8 had HIV infection and did not develop cryptococcosis (the HIV+CN- group), and 15 did not have HIV infection and did not develop cryptococcosis (the HIV- group) (cohort 2). Results. In cohort 1, the percentage of memory B cells that expressed IgM was lower among HIV+CN+ subjects, compared with HIV+CN- subjects (P<.01) and HIV- subjects (P<.05); expression of IgM on =S50{\%} of memory B cells was a significant predictor of C. neoformans disease status (odds ratio, 5.5; P = .03). In cohort 2, the percentage of memory B cells that expressed IgM was lower in HIV+CN+ subjects than in HIV+CNsubjects (P = .02) and HIV- subjects (P<.01); an IgM + memory B cell percentage of =538.5{\%} was a significant predictor of future development of cryptococcosis (odds ratio, 14; P = .02). Conclusions. These findings suggest that HIV-infected persons in whom the percentage of memory B cells that express IgM is decreased might be at greater risk for the development of cryptococcosis.",
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AU - Subramaniam, Krishanthi

AU - Metzger, Brian

AU - Hanau, Lawrence H.

AU - Guh, Alice

AU - Rucker, Lisa

AU - Badri, Sheila

AU - Pirofski, Liise-anne

PY - 2009/7/15

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N2 - Background. The role of B cells in resistance to Cryptococcus neoformans disease (i.e., cryptococcosis) is unknown. Given evidence that IgM + memory B cells are required for immunity to other encapsulated pathogens, we hypothesized that these cells might contribute to resistance to cryptococcosis. Methods. We compared levels of IgM expression on memory B cells in 29 HIV-infected individuals who had a history of cryptococcosis (the HIV+CN+ group) with levels in 30 human immunodeficiency virus (HlV)-infected subjects who had no history of cryptococcosis (the HIV+CN- group) and 20 HIV-uninfected subjects who had no history of cryptococcosis (the HIV- group) (cohort 1). We also determined levels of IgM expression on memory B cells in banked samples obtained before cryptococcosis onset from 31 participants in the Multicenter AIDS Cohort Study, of whom 8 had HIV infection and subsequently developed cryptococcosis (the HIV+CN+ group), 8 had HIV infection and did not develop cryptococcosis (the HIV+CN- group), and 15 did not have HIV infection and did not develop cryptococcosis (the HIV- group) (cohort 2). Results. In cohort 1, the percentage of memory B cells that expressed IgM was lower among HIV+CN+ subjects, compared with HIV+CN- subjects (P<.01) and HIV- subjects (P<.05); expression of IgM on =S50% of memory B cells was a significant predictor of C. neoformans disease status (odds ratio, 5.5; P = .03). In cohort 2, the percentage of memory B cells that expressed IgM was lower in HIV+CN+ subjects than in HIV+CNsubjects (P = .02) and HIV- subjects (P<.01); an IgM + memory B cell percentage of =538.5% was a significant predictor of future development of cryptococcosis (odds ratio, 14; P = .02). Conclusions. These findings suggest that HIV-infected persons in whom the percentage of memory B cells that express IgM is decreased might be at greater risk for the development of cryptococcosis.

AB - Background. The role of B cells in resistance to Cryptococcus neoformans disease (i.e., cryptococcosis) is unknown. Given evidence that IgM + memory B cells are required for immunity to other encapsulated pathogens, we hypothesized that these cells might contribute to resistance to cryptococcosis. Methods. We compared levels of IgM expression on memory B cells in 29 HIV-infected individuals who had a history of cryptococcosis (the HIV+CN+ group) with levels in 30 human immunodeficiency virus (HlV)-infected subjects who had no history of cryptococcosis (the HIV+CN- group) and 20 HIV-uninfected subjects who had no history of cryptococcosis (the HIV- group) (cohort 1). We also determined levels of IgM expression on memory B cells in banked samples obtained before cryptococcosis onset from 31 participants in the Multicenter AIDS Cohort Study, of whom 8 had HIV infection and subsequently developed cryptococcosis (the HIV+CN+ group), 8 had HIV infection and did not develop cryptococcosis (the HIV+CN- group), and 15 did not have HIV infection and did not develop cryptococcosis (the HIV- group) (cohort 2). Results. In cohort 1, the percentage of memory B cells that expressed IgM was lower among HIV+CN+ subjects, compared with HIV+CN- subjects (P<.01) and HIV- subjects (P<.05); expression of IgM on =S50% of memory B cells was a significant predictor of C. neoformans disease status (odds ratio, 5.5; P = .03). In cohort 2, the percentage of memory B cells that expressed IgM was lower in HIV+CN+ subjects than in HIV+CNsubjects (P = .02) and HIV- subjects (P<.01); an IgM + memory B cell percentage of =538.5% was a significant predictor of future development of cryptococcosis (odds ratio, 14; P = .02). Conclusions. These findings suggest that HIV-infected persons in whom the percentage of memory B cells that express IgM is decreased might be at greater risk for the development of cryptococcosis.

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