2-oxo-3-alkynoic acids, universal mechanism-based inactivators of thiamin diphosphate-dependent decarboxylases: Synthesis and evidence for potent inactivation of the pyruvate dehydrogenase multienzyme complex

Angela Brown, Natalia Nemeria, Jizu Yi, Deqi Zhang, William B. Jordan, Rosane S. Machado, John R. Guest, Frank Jordan

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

A new class of compounds, the 2-oxo-3-alkynoic acids with a phenyl substituent at carbon 4 was reported by the authors as potent irreversible and mechanism-based inhibitors of the thiamin diphosphate- (ThDP-) dependent enzyme pyruvate decarboxylase [Chiu, C.-F., and Jordan, F. (1994) J. Org. Chem. 59, 5763-5766]. The method has been successfully extended to the synthesis of the 4-, 5-, and 7-carbon aliphatic members of this family of compounds. These three compounds were then tested on three ThDP-dependent pyruvate decarboxylases: the Escherichia coli pyruvate dehydrogenase multienzyme complex (PDHc) and its El (ThDP-dependent) component, pyruvate oxidase (POX, phosphorylating; from Lactobacillus plantarum), and pyruvate decarboxylase (PDC) from Saccharomyces cerevisiae. All three enzymes were irreversibly inhibited by the new compounds. The 4-carbon acid is the best substrate-analog inactivator known to date for PDHc, more potent than either fluoropyruvate or bromopyruvate. The following conclusions were drawn from extensive studies with PDHc: (a) The kinetics of inactivation of PDH complexes and of resolved E1 by 2-oxo-3-alkynoic acids is time- and concentration-dependent. (b) The 4-carbon acid has a K(i) 2 orders of magnitude stronger than the 5-carbon acid, clearly demonstrating the substrate specificity of PDHc. (c) The rate of inactivation of PDH complexes and of resolved El by 2-oxo-3-alkynoic acids is enhanced by the addition of ThDP and MgCl2. (d) Pyruvate completely protects El and partially protects PDHc from inactivation by 2-oxo-3-butynoic acid. (e) E1 but not E2-E3 is the target of inactivation by 2-oxo-3-butynoic acid. (f) inactivation of E1 by 2- oxo-3-butynoic acid is accompanied by modification of 1.3 cysteines/E1 monomer. The order of reactivity with the 4-carbon acid was PDHc > POX > PDC. While the order of reactivity with PDHc and POX was 2-oxo-3-butynoic acid > 2-oxo-3-pentynoic acid > 2-oxo-3-heptynoic acid, the order of reactivity was reversed with PDC.

Original languageEnglish (US)
Pages (from-to)8071-8081
Number of pages11
JournalBiochemistry
Volume36
Issue number26
DOIs
StatePublished - Jul 1 1997
Externally publishedYes

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Multienzyme Complexes
Thiamine Pyrophosphate
Pyruvate Dehydrogenase Complex
Carboxy-Lyases
Pyruvic Acid
Oxidoreductases
Pyruvate Decarboxylase
Acids
Carbon
Pyruvate Oxidase
Lactobacillus plantarum
Jordan
Magnesium Chloride
Substrates
Enzymes
Substrate Specificity
Yeast
Escherichia coli
Cysteine
Saccharomyces cerevisiae

ASJC Scopus subject areas

  • Biochemistry

Cite this

2-oxo-3-alkynoic acids, universal mechanism-based inactivators of thiamin diphosphate-dependent decarboxylases : Synthesis and evidence for potent inactivation of the pyruvate dehydrogenase multienzyme complex. / Brown, Angela; Nemeria, Natalia; Yi, Jizu; Zhang, Deqi; Jordan, William B.; Machado, Rosane S.; Guest, John R.; Jordan, Frank.

In: Biochemistry, Vol. 36, No. 26, 01.07.1997, p. 8071-8081.

Research output: Contribution to journalArticle

Brown, Angela ; Nemeria, Natalia ; Yi, Jizu ; Zhang, Deqi ; Jordan, William B. ; Machado, Rosane S. ; Guest, John R. ; Jordan, Frank. / 2-oxo-3-alkynoic acids, universal mechanism-based inactivators of thiamin diphosphate-dependent decarboxylases : Synthesis and evidence for potent inactivation of the pyruvate dehydrogenase multienzyme complex. In: Biochemistry. 1997 ; Vol. 36, No. 26. pp. 8071-8081.
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AU - Brown, Angela

AU - Nemeria, Natalia

AU - Yi, Jizu

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AU - Guest, John R.

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