13C NMR assignments of the protonated carbons of [d(TAGCGCTA)]2 by two-dimensional proton-detected heteronuclear correlation.

S. R. LaPlante, J. Ashcroft, David Cowburn, G. C. Levy, P. N. Borer

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

The resonances of the protonated carbons of [d(TAGCGCTA)]2 have been assigned by the two-dimensional proton-detected double-quantum heteronuclear correlation experiment [( 1H-13C]-DQCOSY). 13C-coupled and 13C-decoupled versions of the experiment were used. The assignment method is discussed in detail. The deoxyribose cross peaks segregate into five well-resolved regions, and the base cross peaks have distinct features that are helpful for assignments. The cross peaks from the 1H-13C pairs at the Cyd5, Ado2 and ThdCH3 base positions fall in separate regions of the spectrum from each other; they also are resolved from the closely spaced Ado8, Guo8, Cyd6 and Thd6. Additional parameters for distinction of the base signals are their differing J-coupling values and long-range coupling patterns.

Original languageEnglish (US)
Pages (from-to)1089-1099
Number of pages11
JournalJournal of biomolecular structure & dynamics
Volume5
Issue number5
StatePublished - Apr 1988
Externally publishedYes

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Deoxyribose
Protons
Carbon
Carbon-13 Magnetic Resonance Spectroscopy

ASJC Scopus subject areas

  • Molecular Biology

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13C NMR assignments of the protonated carbons of [d(TAGCGCTA)]2 by two-dimensional proton-detected heteronuclear correlation. / LaPlante, S. R.; Ashcroft, J.; Cowburn, David; Levy, G. C.; Borer, P. N.

In: Journal of biomolecular structure & dynamics, Vol. 5, No. 5, 04.1988, p. 1089-1099.

Research output: Contribution to journalArticle

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AU - Borer, P. N.

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