TY - JOUR
T1 - δ- and β-crystallin mRNA levels in the embryonic and posthatched chicken lens
T2 - Temporal and spatial changes during development
AU - Hejtmancik, J. Fielding
AU - Beebe, David C.
AU - Ostrer, Harry
AU - Piatigorsky, Joram
PY - 1985/5
Y1 - 1985/5
N2 - The levels of δ- and β-crystallin mRNAs were examined by cDNA hybridization in the embryonic and posthatched chicken eye lens. Four different cloned β-crystallin cDNAs were used, allowing discrimination among different members of the β-crystallin family. Each crystallin mRNA displayed a characteristic temporal and spatial pattern in the developing lens. δ-Crystallin mRNA accumulated rapidly during early embryonic development; by contrast, the β-crystallin mRNAs began to accumulate rapidly near the end of embryogenesis. Both δ- and β-crystallin mRNAs increased in the lens for the first month after hatching and began to decrease 3 months after hatching. The levels of the δ- and the different β-crystallin mRNAs were also differentially regulated in cultured embryonic lens epithelia. The most fiber cell specific crystallin gene product in the differentiating lens was the β35 mRNA. These experiments provide a quantitative basis for exploring the differential expression of the δ- and β-crystallin gene families in the chicken lens.
AB - The levels of δ- and β-crystallin mRNAs were examined by cDNA hybridization in the embryonic and posthatched chicken eye lens. Four different cloned β-crystallin cDNAs were used, allowing discrimination among different members of the β-crystallin family. Each crystallin mRNA displayed a characteristic temporal and spatial pattern in the developing lens. δ-Crystallin mRNA accumulated rapidly during early embryonic development; by contrast, the β-crystallin mRNAs began to accumulate rapidly near the end of embryogenesis. Both δ- and β-crystallin mRNAs increased in the lens for the first month after hatching and began to decrease 3 months after hatching. The levels of the δ- and the different β-crystallin mRNAs were also differentially regulated in cultured embryonic lens epithelia. The most fiber cell specific crystallin gene product in the differentiating lens was the β35 mRNA. These experiments provide a quantitative basis for exploring the differential expression of the δ- and β-crystallin gene families in the chicken lens.
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U2 - 10.1016/0012-1606(85)90347-1
DO - 10.1016/0012-1606(85)90347-1
M3 - Article
C2 - 3987967
AN - SCOPUS:0021874994
SN - 0012-1606
VL - 109
SP - 72
EP - 81
JO - Developmental Biology
JF - Developmental Biology
IS - 1
ER -