α-1 Adrenergic receptors stimulation induces the proliferation of neural progenitor cells in vitro

Takeshi Hiramoto, Yoshiaki Ihara, Yasuhiro Watanabe

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

The proliferation of neural progenitor cells (NPCs) is regulated by classical neurotransmitters such as dopamine, serotonin and acetylcholine, via its own receptors. Previous studies have reported that the depletion of l-norepinephrine decreases the proliferation of NPCs in the adult rat hippocampus and it has been suggested that l-norepinephrine regulates the proliferation of NPCs. However, it remains unknown whether or not adrenergic receptors are involved in the increased proliferation of NPCs. In the present study, an MTT cell proliferation assay was carried out in order to investigate the roles played by adrenergic receptors in the proliferation of NPCs. We demonstrated that l-epinephrine enhanced the proliferation of embryonic NPCs in vitro. In addition, the α-1 adrenergic receptor agonist l-phenylephrine was found to enhance the proliferation of NPCs, whereas an α-adrenergic antagonist and selective α-1 antagonists significantly inhibited cell proliferation increases induced by l-epinephrine and l-phenylephrine. These results suggest that stimulation with α-1 adrenergic receptors induces the proliferation of embryonic NPCs.

Original languageEnglish (US)
Pages (from-to)25-28
Number of pages4
JournalNeuroscience Letters
Volume408
Issue number1
DOIs
StatePublished - Nov 6 2006
Externally publishedYes

Fingerprint

Adrenergic Receptors
Stem Cells
Phenylephrine
Epinephrine
Norepinephrine
Cell Proliferation
Adrenergic Agonists
Adrenergic Antagonists
In Vitro Techniques
Acetylcholine
Neurotransmitter Agents
Hippocampus
Dopamine
Serotonin

Keywords

  • α-1 Adrenergic receptors
  • Cell proliferation
  • l-Epinephrine
  • l-Phenylephrine
  • Neural progenitor cells

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

α-1 Adrenergic receptors stimulation induces the proliferation of neural progenitor cells in vitro. / Hiramoto, Takeshi; Ihara, Yoshiaki; Watanabe, Yasuhiro.

In: Neuroscience Letters, Vol. 408, No. 1, 06.11.2006, p. 25-28.

Research output: Contribution to journalArticle

Hiramoto, Takeshi ; Ihara, Yoshiaki ; Watanabe, Yasuhiro. / α-1 Adrenergic receptors stimulation induces the proliferation of neural progenitor cells in vitro. In: Neuroscience Letters. 2006 ; Vol. 408, No. 1. pp. 25-28.
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