TIME RESOLVED SYNCHROTRON XRAY FOOTPRINTING OF NUCLEIC ACIDS

  • Brenowitz, Michael D. (PI)
  • Chance, Britton (PI)
  • Miller, Lisa (PI)
  • Blasie, J. Kent (PI)
  • Maleknia, Simin (PI)
  • Goldsmith, Sharon (PI)
  • Pyle, Anna Marie (PI)
  • Toomey, John R. (PI)
  • Franzen, Stephen (PI)
  • Downard, Kevin (PI)
  • Marinkovic, Nebojsa (PI)
  • Sperber, Ellen (PI)
  • Crothers, Donald (PI)
  • Penner-Hahn, James E. (PI)
  • Kravtsov, Irina (PI)
  • Davies, David (PI)
  • Silva, Alberto (PI)
  • Morris, Ann-Tabor (PI)
  • Hurley, J.I.M. (PI)
  • Fisher, Timothy (PI)
  • Seeman, Nadrian C. (PI)
  • Burke, John (PI)
  • Peter, Sun (PI)
  • Wlodawer, Alex (PI)
  • Studier, Bill (PI)
  • Xinhua, Ji (PI)
  • Tran, Alex (PI)
  • Dauter, Zbigniew (PI)
  • Honggao, Yan (PI)
  • Kovacs, Kevin (PI)
  • vanamee, Eva (PI)
  • Snyder, Gary (PI)
  • Que Lawrence, [No Value] (PI)
  • Chen, Terry (PI)
  • Pedersen, Lars (PI)
  • Hurley, James (PI)
  • Sonenberg, Nils (PI)
  • Anderson, Richard (PI)
  • Bubacco, Luigi (PI)
  • Sharpe, L.E.E. (PI)
  • Kallenbach, Neville (PI)
  • Aihua, Xie (PI)
  • Caffrey, Martin (PI)
  • Sinclair, Robert (PI)
  • Woodson, Sarah (PI)
  • Kwok, Yue (PI)
  • Blasie, J. Kent (PI)
  • Lawrence, Que (PI)
  • Heiney, Paul (PI)
  • Anni, Helen (PI)
  • Holmes, Kenneth (PI)
  • Powers, Linda (PI)
  • Scheuring, E.V.A. (PI)
  • Scarrow, Robert (PI)
  • Chance, Mark (PI)
  • Sagi, Irit (PI)
  • Maroney, Michael (PI)
  • Sullivan, Michael (PI)
  • Caroline Caroline, Lee (PI)
  • Penner-Hahn, James E. (PI)
  • Blasie, J. Kent (PI)
  • Hurley, J.I.M. (PI)
  • Seeman, Nadrian C. (PI)
  • Toomey, John R. (PI)
  • Que Lawrence, [No Value] (PI)
  • Bubacco, Luigi (PI)
  • Sharpe, L.E.E. (PI)
  • Kallenbach, Neville (PI)
  • Aihua, Xie (PI)
  • Caffrey, Martin (PI)
  • Sinclair, Robert (PI)
  • Woodson, Sarah (PI)
  • Kwok, Yue (PI)
  • Blasie, J. Kent (PI)
  • Lawrence, Que (PI)
  • Heiney, Paul (PI)
  • Anni, Helen (PI)
  • Holmes, Kenneth (PI)
  • Powers, Linda (PI)
  • Scheuring, E.V.A. (PI)
  • Scarrow, Robert (PI)
  • Chance, Mark (PI)
  • Sagi, Irit (PI)
  • Maroney, Michael (PI)
  • Sullivan, Michael (PI)
  • Caroline Caroline, Lee (PI)

Project: Research project

Project Details

Description

Hydroxyl radicals (*OH) can cleave the phosphodiester backbone of
nucleic acids and are valuable reagents in the study of nucleic acid
structure and protein-nucleic acid interactions through [unreadable]footprinting[unreadable]
studies. Irradiation of solutions by high flux "white light" x-ray
beams based on bending magnet beamlines at the National Synchrotron
Light Source (NSLS) yields sufficient concentrations of *OH so that
quantitative nuclease protection ("foot-printing") studies of DNA and
RNA can be conducted with 10 [unreadable] 20 msec x-ray exposure. The utility of
this timescale for nucleic acid cleavage is demonstrated by
synchrotron x-ray time-resolved "footprinting" analyses of the Mg2+
-dependent folding of the Tetrahymena thermo-philia L-21 Sca I
ribozyme RNA. Since RNA folding progress curves over timescales from
msec to minutes have been obtained for 25 regions of the ribozyme,
this research provides an unprecedented structural kinetic picture of
the folding of a large macromolecule. Synchro-tron x-ray
"footprinting" is a new approach of general applicability for the
study of time-re-solved structural changes of nucleic acid
conformation and protein-nucleic acid complexes.
StatusFinished
Effective start/end date10/1/978/31/99

Funding

  • National Center for Research Resources

ASJC

  • Medical Laboratory Technology
  • Radiation
  • Genetics
  • Surfaces, Coatings and Films
  • Molecular Biology
  • Structural Biology
  • Biophysics
  • Management of Technology and Innovation
  • Research and Theory
  • Spectroscopy
  • Biochemistry
  • Organizational Behavior and Human Resource Management
  • Microbiology
  • Physical and Theoretical Chemistry
  • Computational Mechanics

Fingerprint Explore the research topics touched on by this project. These labels are generated based on the underlying awards/grants. Together they form a unique fingerprint.