TAXOL EFFECTS ON SIGNALING PATHWAYS IN OVARIAN CELLS

Project: Research project

Project Details

Description

DESCRIPTION

Taxol is a natural product which has significant antitumor activity in a
large number of human tumors. It has recently become appreciated that in
addition to stabilizing microtubules and blocking cell cycle progress, taxol
can alter gene expression by interacting with the MAP-kinase signal
transduction pathway. Previous studies from the foreign and US PIs had
demonstrated that tyrosine phosphorylation of the adaptor protein, Shc, and
formation of Shc/grb2/SOS complexes occurs in a taxol-treated murine
macrophage cell line. The investigators propose to extend these
observations to examine human ovarian carcinoma cells. The specific aims
are to: 1. Study the effect of taxol on activation of the MAP-kinase
pathway in human ovarian carcinoma cell lines; 2. Study the effect of
taxotere and other taxol analogs compared to that of taxol on the activation
of the Ras/MAPK signaling pathway and the relationship of this activity to
effects on microtubule stabilization and cytotoxicity; 3. Compare taxol and
taxotere in MAPK signaling pathway in taxol-sensitive and taxol-resistant
human ovarian carcinoma cells.
StatusFinished
Effective start/end date3/1/982/28/99

Funding

  • Fogarty International Center

ASJC

  • Cancer Research
  • Cell Biology
  • Toxicology
  • Pharmacology
  • Immunology

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