STUDIES ON A CALCIUM SENSING RECEPTOR

Project: Research project

Project Details

Description

Brown and colleagues (Nature 1993) have cloned a novel calcium-sensing
receptor (CaR) which is a member of the G protein-coupled receptor
superfamily similar in structure to the metabotropic glutamate receptors.
The CaR is expressed in a limited range of cell types including kidney,
brain, thyroid C cells, and most prominently parathyroid cells.
Mutations in the CaR identified in the human genetic disorder familial
hypocalciuric hypocalcemia provide strong evidence for the physiologic
importance of the receptor in calcium sensing generally and in regulation
of parathyroid hormone secretion by extracellular ionized calcium in
particular. We are studying several aspects of the receptor's structure
and function. We have raised polyclonal antisera to several synthetic
peptides corresponding to sequences in the large extracellular domain.
These recognized the receptor on western blots and should be useful for
a wide variety of studies. Development of monoclonal antibodies is
planned with the long-range goal of being used as imaging reagents for
parathyroid tumors in patients with failed neck explorations. We are
also studying the coupling of the receptor to G proteins using
transfection techniques, and by defining the normal complement of G
protein alpha subunits in parathyroid cells.
StatusNot started

ASJC

  • Nephrology