SOMATIC CELL GENETICS OF IG GENES

Project: Research project

Project Details

Description

The somatic cell genetics of the instability of immunoglobulin genes in
cultured hybridoma and myeloma cells will be studied. Earlier studies had
shown that mutants with changes in the variable and constant regions of
immunoglobulins arise frequently and spontaneously in cultured cells. The
molecular and genetic mechanisms of these events will be examined by
determining the relative rates of V and C region mutations and the base
changes that occur in these mutants. Similar mutants will be sought in
vivo.

Mutants with both increases and decreases in antigen binding and changes in
effector functions will be identified. The structural basis of these
changes will be determined and the information gained will be used to
construct antibodies that could be more effective reagents in protection
against infection, neutralization of toxins, and targeting to tumors.
StatusFinished
Effective start/end date1/1/901/1/90

Funding

  • National Cancer Institute

ASJC

  • Genetics

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