SIGNAL TRANSDUCTION &ACTIN DURING AMOEBOID CHEMOTAXIS

Project: Research project

Project Details

Description

This project has as its general goal the study of cell motility and chemotaxis in cultured
cells in vitro in response to exogenous growth factors such as EGF.The results during the
previous funding period implicate cofilin in controlling sites of actin polymerization and protrusion
in cultured cells. This places cofilin in the unexpected role of a decision maker during chemotaxis
helping to determine cell direction in response to chemoattractant. In addition, these results
implicate N-WASP as a key regulator of chemotaxis and suggest that cofilin and N-WASP
cooperate to regulate cell direction and that both are acting during the earliest barbed end
transient in response to EGF.These observations suggest that N-WASP might contribute to
chemotaxis through specialized structures at the leading edge that affect adhesion. To evaluate
this idea, much more work needs to be done to define the N-WASP dependent actin filament
compartment at the leading edge of lamellipods, and its relationship, in space and time, to cofilin
activity, upstream regulators of N-WASP, and focal complex formation. The challenge for the
future is to understand how cofilin is regulated with high spatial and temporal precision as part of
the steering wheel of the cell and how the cofilin regulatory cycle interacts with N-WASP to cause
directed cell movement and chemotaxis in culture.
StatusFinished
Effective start/end date1/1/9012/31/09

Funding

  • National Institute of General Medical Sciences
  • National Institute of General Medical Sciences
  • National Institute of General Medical Sciences
  • National Institute of General Medical Sciences
  • National Institute of General Medical Sciences

ASJC

  • Genetics
  • Cell Biology

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