Project: Research project

Project Details


The identification of cellular oncogenes has allowed us the opportunity to
define the malignant process and cellular differentiation within the
context of a relatively small subset of genes. We have been studying the
role of the c-myc oncogene in the differentiation of mouse erythroleukemia
(MEL) cells. The major goal of this proposal is to study the mechanisms by
which the c-myc regulation we have observed results in the terminal
erythroid differentiation of MEL cells. Our approach will be to transfect
MEL cells with c-myc clones attached to the mouse metallotionein I promoter
and to study the effects of independently regulated c-myc on MEL cell

We also will determine whether the changes we have observed in c-myc mRNA
levels during MEL cell differentiation are due to transcriptional or
post-transcriptional controls. If control is exerted on the
transcriptional level, we will be able to plan future experiments to
determine the DNA sequences responsible for the rapid c-myc mRNA changes we
have observed in MEL cells.

Another aspect of MEL cell differentiation we will investigate is the
relationship between DNA synthesis and cellular differentiation. We will
relate these parameter to c-myc regulation in an attempt to clarify a
controversy regarding the necessity for DNA synthesis in MEL cell

Finally, we will study the role of other cellular oncogenes in the MEL cell
differentiation process. Eventually, we hope to perform MEL cell
transfections using other oncogenes in order to demonstrate relationships
between c-myc regulation, the expression of other cellular oncogenes, and
MEL cell differentiation.

The purpose of these experiments is to further understand the link between
transformed and differentiated states in terms of the regulation of
cellular oncogenes. (X)
Effective start/end date12/31/8912/31/89


  • Molecular Biology
  • Genetics


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