RNA TRANSPORT AND LOCALIZATION IN YEAST IN SITU

Project: Research project

Project Details

Description

DESCRIPTION (Adapted from the applicant's abstract): In this application,
the P.I. proposes a series of aims designed to address the localization of
ASH1 mRNA in yeast cells. Ash1p is an asymmetrically localized protein,
important in repressing mating type switching in the daughter cell. The
asymmetry of this nuclear protein results from the localization of its mRNA
at the distal cortex of a daughter cell, the bud, and the greater
accumulation of this negative regulator of gene expression in daughter
nuclei. In the first aim, cis-acting sequences and trans-acting factors
will be isolated in order to determine the protein components of the
mechanism. Specific aim 2 proposes experiments to follow the transport,
export and localization of this RNA from the site of its transcription to
its final location at the cortex of the bud. In the final specific aim, the
mechanism of nuclear export will be assessed to determine points in the
pathway where rate-limitations may exist.
StatusFinished
Effective start/end date1/1/9812/31/98

Funding

  • National Institute of General Medical Sciences

ASJC

  • Genetics
  • Molecular Biology
  • Cell Biology
  • Histology
  • Microbiology

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