PHOSPHORIMAGER TM IMAGE ANALYSIS SYSTEM

Project: Research project

Project Details

Description

We are requesting an image analysis system composed of a two-dimensional
spatially-restricted radioactivity detector (Molecular Dynamics
PhosphorImager(TM)), an ImageQuant workstation and a Sun UNIX workstation
in order to enhance the detection of radioisotopically labeled molecules
resolved by electrophoretic and chromatographic separations. In
particular, the acquisition of the PhosphorImager(TM) will enhance our
ability to conduct quantitative analysis of these separations. When
compared to conventional autoradiography, the unique features of this
instrument are twenty to one hundred fold increased sensitivity and a five
orders of magnitude linear response to radioactivity. These features are
accomplished with high spatial resolution, a fact critical to many of the
proposed applications. Availability of this instrument to individual
investigations will be maximized by the incorporation of the
PhosphorImager(TM) into our existing computer network. The images files
acquired by the requested PhosphorImager(TM) will be exported both to the
requested image analysis workstations and to additional workstations
located within departmental computer facilities and individual
laboratories. Structuring the image analysis facility in this way will
allow investigators to conduct detailed and rigorous analyses of their
electrophoretograms and chromatograms.
StatusFinished
Effective start/end date9/1/9211/30/94

Funding

  • National Center for Research Resources

ASJC

  • Signal Processing
  • Radiation

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