PEPTIDE SYNTHESIS &SEQUENCE - CIRCULAR DICHROISM

We are requesting instruments to enhance the analytical and synthetic
capabilities of the Laboratory for Macromolecular Analysis (LMA) at Albert
Einstein College of Medicine (AECOM). Our current peptide synthesizer and
protein sequencer are already used at full capacity. We request an
additional, more flexible, peptide synthesizer for structure-function
analysis and biological applications of peptides in research projects in
endocrinology, immunology, and protein-membrane interactions. An
additional protein sequencer is needed to meet the demand created by new
projects from current investigators, projects from newly hired faculty, as
well as a novel use of this instrument. A sensitive circular dichroism
(CD) spectropolarimeter is requested for a variety of structure-function
studies. Capillary electrophoresis instrumentation will enhance our other
analytical services by providing rapid purity checks on our synthetic
peptides as well as peptides and proteins submitted for sequence analysis,
a real-time analysis of peptide digests, and development of assays for
peptide hormone processing and secretion. These instruments, together with
others already functioning in the LMA, will enhance the ability of AECOM
investigators to incorporate protein structural analysis into their
research programs.