MOLECULAR CHARACTERIZATION OF C NEOFORMANS ANTIBODIES

The fungus Cryptococcus neoformans causes life-threatening infections in
approximately 10% of all patients with AIDS. A considerable body of
evidence indicates that antibody to the cryptococcal capsular
polysaccharide can contribute to host defenses. We have identified
monoclonal antibodies to the capsular polysaccharide antigens. The
experimental approach will involve: the study of the molecular structure
of individual anticryptococcal antibodies are glycosylated in their
antigen binding regions; and the study of serum antibody populations
conjugate immunization using rabbit anti-idiotypic reagents and
isoelectric focusing. These studies will produce information of
fundamental interest in immunology and in the antibody response to fungal
antigens. The studies in mice will pave the way for the molecular
characterization of the human antibody response. These studies will also
produce important information for the development of antibodies of C.
neoformans for human therapy and vaccines against this fungal pathogen.