MOLECULAR ANALYSIS OF CHEMOTAXIS IN METASTATIC CELLS

Project: Research project

Project Details

Description

Significant advances in understanding amoeboid chemotaxis at
the molecular level have been made recently using relatively simple model
systems that can be manipulated in culture. One of these, Dictyostelium
discoideum, has been particularly valuable in analysis of mechanisms
involved in agonist regulated pseudopod extension and chemotaxis.
Molecular probes and assays developed with this model have been directly
applicable to mammalian cell chemotaxis. We propose to dissect, at the
molecular level, the signal transduction pathway which regulates
pseudopod formation and chemotaxis in Lewis lung carcinoma cells. In
these preliminary studies we will apply the Dictyostelium paradigm for
this pathway directly to M27 cells using molecular probes and assays
already developed for Dictyostelium. We will test the relationship
between receptor mediated actin nucleation activity and cell motility
events which are involved in several key steps of metastasis. The roles
of several candidate proteins in these steps will be evaluated.
StatusFinished
Effective start/end date7/1/926/30/94

Funding

  • National Cancer Institute

ASJC

  • Cell Biology

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