Leukemogenesis by Murine Retroviruses

Project: Research project

Project Details

Description

DESCRIPTION (provided by applicant): Murine leukemia viruses (MuLVs) are potent
inducers of hematopoietic tumors in mice. Transcriptional regulatory sequences
within the long terminal repeats (LTRs) of these viruses control the expression
of the viral genomes and play crucial roles in the process of tumorigenesis by
these viruses. They determine the type of tumor that specific MuLVs cause, they
influence the ability of the viruses to infect the target cells for disease,
and they play a role in the tumorigenic activation of cellular genes following
adjacent insertion of the viral genomes. Experiments are proposed here to
compare the transcriptional capacity of different MuLVs on templates that are
integrated at various sites in the mouse genome. These experiments will use
cells from the lineages in which these viruses cause tumors. A second line of
experiments is proposed to identify the cellular genes that are activated by
these viruses in tumors and to begin to test how these genes participate in
tumorigenesis. High-throughput technology is now available that allows such
genetic screens to be performed far more rapidly than in the past. This
approach will be applied to two separate MuLV tumor systems. The genes that are
identified in these screens are expected to provide insight about important and
perhaps unanticipated molecular mechanisms that contribute to the generation of
leukemias and lymphomas.
StatusFinished
Effective start/end date1/1/9011/30/06

Funding

  • National Cancer Institute
  • National Cancer Institute
  • National Cancer Institute: $371,575.00
  • National Cancer Institute
  • National Cancer Institute: $290,099.00

ASJC

  • Genetics
  • Molecular Biology
  • Cancer Research
  • Virology

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