JEOL 100S FOR MEMBRANE CYTOSKELETAL STUDIES

  • Satir, Birgit H. (PI)

Project: Research project

Project Details

Description

This grant will provide a "user-friendly" electron microscope for research
in cell biology by a core group of cell biologists and other users. Some
of the questions being asked in the research involved include: 1) function
and assembly of specific intramembrane particle arrays during secretion
using freeze fracture electron microscopy and mutant analysis; 2) structure
and function of cilia, including the transmission electron microscopy of
sliding microtubules and dynein arm morphology; 3) actin filament
interaction with the cell membrane and the localization in the cell of two
actin binding proteins using IgG-gold immunocytochemistry at the electron
microscope level; 4) electron microscope analysis of H. Halobium strains
genetically altered in photoenergy conversion and photosensory signaling by
the rhodopsin-like molecules in their membranes. 5) Studies to probe the
differential compartmentalization and sorting of two polypeptides
somatostatin, a secretory protein and vesicular stomatitis virus, a
membrane protein to determine where in the secretory pathway the two
separate using IgG-gold immunocytochemistry at the electron microscope
level.
StatusFinished
Effective start/end date12/1/8411/30/85

ASJC

  • Medicine(all)