ISOLATION AND CHARACTERIZATION OF THE GENE ENCODING OKM5

Project: Research project

Project Details

Description

This is a proposal to isolate and characterize the gene for OKM5
and to study the function of the encoded protein. This gene
encodes a human macrophage/endothelial cell surface molecule
which serves as a differentiation marker for these cells and may
be involved in the cytoadherence of lymphocytes to endothelial
cells. This involvement may help in the understanding of how
malignant cells metastasize. A cDNA clone for the antigen using
the mouse transformant which I have already selected will be
isolated. The sequence of this cDNA will be determined to
deduce the protein structure. The function of the OKM5 molecule
will be studied using immunologic blocking methods and somatic
cell genetic techniques. Expression of the antigen on the surface
of a variety of human promonocytic tumor cell lines is induced by
a variety of factors. These inducible systems provide a model for
the study of the regulation of the gene.
StatusFinished
Effective start/end date1/1/901/1/90

Funding

  • National Cancer Institute

ASJC

  • Cell Biology

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