HEPATIC UPTAKE AND TRANSPORT MECHANISMS

Project: Research project

Project Details

Description

Although specific liver cell plasma membrane (LPM) receptor proteins have
been implicated in ligand uptake by the liver, the determinants of their
function and the influence of other LPM constituents such as carbohydrates
and lipids are unclear. Previous studies of LPM receptor function have
been performed in systems in which receptors have been purified, removing
them from their normal milieu, or in isolated membranes or cells in which
normal cellular architecture has been destroyed. The isolated perfused rat
liver enables these studies to be performed in a viable, intact organ free
of hemodynamic and hormonal alterations which may complicate interpretation
of studies performed in intact animals. The specific aims of the proposed
research are: (1) to study the effect of specific modifications to the LPM
surface on transport of organic anions and asialoglycoproteins, (2) to
study the role of specific cytoskeletal elements (microtubules,
microfilaments, and calmodulin) on hepatic transport of asialoglycoproteins
and organic anions, (3) to study the possible role of the
asialoglycoprotein receptor in hepatic uptake and biliary excretion of
polymeric IgA and (4) to define normal uptake kinetics of other ligands
which may have receptor-mediated uptake mechanisms. These ligands will
include copper, lipoproteins, protoporphyrins and bilirubin photoisomers.
StatusFinished
Effective start/end date4/1/836/30/86

Funding

  • National Institutes of Health

ASJC

  • Medicine(all)
  • Biophysics
  • Physiology
  • Biochemistry
  • Cell Biology
  • Hepatology

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